Overview

  • Product name

  • Description

    Rabbit polyclonal to CIP2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CIP2A aa 550-600. The exact sequence is proprietary.

  • Positive control

    • Human colon carcinoma. Human ovarian carcinoma.
  • General notes

    Concentration is optimized for IHC and not determined

    Protein previously labeled as p90 Autoantigen.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
  • Tissue specificity

    Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
  • Cellular localization

    Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancerous inhibitor of PP2A antibody
    • Cancerous Inhibitor of Protein Phosphatase 2A antibody
    • CIP2A antibody
    • CIP2A_HUMAN antibody
    • FLJ12850 antibody
    • Kiaa1524 antibody
    • MGC163436 antibody
    • p90 antibody
    • p90 autoantigen antibody
    • Protein CIP2A antibody
    see all

Images

  • Detection of Human CIP2A by Immunohistochemistry.Sample: FFPE section of Human colon carcinoma.Antibody: ab84547 used at a dilution of 1/250.Detection: DAB staining.

  • Detection of Human CIP2A by Immunohistochemistry.Sample: FFPE section of Human ovarian carcinoma.Antibody: ab84547 used at a dilution of 1/250.Detection: DAB staining.

  • Immunohistochemical analysis of Human breast tumour tissue, staining CIP2A with ab84547 at 1/100 dilution.

References

This product has been referenced in:

  • Xu J  et al. Overexpression of S100A4 Predicts Migration, Invasion, and Poor Prognosis of Hypopharyngeal Squamous Cell Carcinoma. Mol Diagn Ther N/A:N/A (2019). Read more (PubMed: 30868407) »
  • Liu CY  et al. Targeting SET to restore PP2A activity disrupts an oncogenic CIP2A-feedforward loop and impairs triple negative breast cancer progression. EBioMedicine 40:263-275 (2019). Read more (PubMed: 30651219) »
See all 9 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Antigen retrieval step
None
Permeabilization
No
Specification
Breast
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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