Anti-CIP2A antibody (ab99518)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-CIP2A antibody
See all CIP2A primary antibodies -
Description
Rabbit polyclonal to CIP2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide within Human CIP2A aa 853-903. The exact sequence is proprietary.
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Positive control
- HeLa and 293T cell lines This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDAMB231
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General notes
Protein previously labeled as p90 Autoantigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab99518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 102 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. -
Tissue specificity
Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level). -
Cellular localization
Membrane. Cytoplasm. Slightly concentrates in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 57650 Human
- Entrez Gene: 224171 Mouse
- Entrez Gene: 360711 Rat
- Omim: 610643 Human
- SwissProt: Q8TCG1 Human
- SwissProt: Q8BWY9 Mouse
- Unigene: 591308 Human
- Unigene: 24491 Mouse
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Alternative names
- Cancerous inhibitor of PP2A antibody
- Cancerous Inhibitor of Protein Phosphatase 2A antibody
- CIP2A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIP2A antibody (ab99518)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CIP2A with ab99518 at 1/200 (1µg/ml). Detection: DAB.
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Immunocytochemistry/ Immunofluorescence - Anti-CIP2A antibody (ab99518)ab99518 stained MDAMB231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99518 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-CIP2A antibody (ab99518)All lanes : Anti-CIP2A antibody (ab99518) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 3 seconds -
Immunoprecipitation - Anti-CIP2A antibody (ab99518)Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99518 at 3 µg/mg lysate; 20% of immunoprecipitate loaded
Western Blot: CIP2A (ab99518) at 0.1 µg/ml
developed using the ECL technique
Exposure time : 3 seconds
Predicted band size : 102 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Williams AP et al. Investigation of PP2A and Its Endogenous Inhibitors in Neuroblastoma Cell Survival and Tumor Growth. Transl Oncol 12:84-95 (2019). Read more (PubMed: 30286326) »
- Stafman LL et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). Read more (PubMed: 30969990) »
