Anti-CIP2A antibody (ab99518)
Key features and details
- Rabbit polyclonal to CIP2A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CIP2A antibody
See all CIP2A primary antibodies -
Description
Rabbit polyclonal to CIP2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide within Human CIP2A aa 853-903. The exact sequence is proprietary.
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General notes
Protein previously labeled as p90 Autoantigen.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab99518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 102 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. -
Tissue specificity
Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level). -
Cellular localization
Membrane. Cytoplasm. Slightly concentrates in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 57650 Human
- Entrez Gene: 224171 Mouse
- Entrez Gene: 360711 Rat
- Omim: 610643 Human
- SwissProt: Q8TCG1 Human
- SwissProt: Q8BWY9 Mouse
- Unigene: 591308 Human
- Unigene: 24491 Mouse
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Alternative names
- Cancerous inhibitor of PP2A antibody
- Cancerous Inhibitor of Protein Phosphatase 2A antibody
- CIP2A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIP2A antibody (ab99518)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CIP2A with ab99518 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-CIP2A antibody (ab99518)All lanes : Anti-CIP2A antibody (ab99518) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 3 seconds -
Immunoprecipitation - Anti-CIP2A antibody (ab99518)Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99518 at 3 µg/mg lysate; 20% of immunoprecipitate loaded
Western Blot: CIP2A (ab99518) at 0.1 µg/ml
developed using the ECL technique
Exposure time : 3 seconds
Predicted band size : 102 kDa
Protocols
Datasheets and documents
References (5)
ab99518 has been referenced in 5 publications.
- Williams AP et al. Investigation of PP2A and Its Endogenous Inhibitors in Neuroblastoma Cell Survival and Tumor Growth. Transl Oncol 12:84-95 (2019). PubMed: 30286326
- Stafman LL et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). PubMed: 30969990
- Chiarini A et al. Over Expressed TKTL1, CIP-2A, and B-MYB Proteins in Uterine Cervix Epithelium Scrapings as Potential Risk Predictive Biomarkers in HR-HPV-Infected LSIL/ASCUS Patients. Front Oncol 9:213 (2019). PubMed: 31001477
- Yin J et al. Cip2a/miR-301a feedback loop promotes cell proliferation and invasion of triple-negative breast cancer. J Cancer 10:5964-5974 (2019). PubMed: 31762806
- Garner EF et al. FTY720 Decreases Tumorigenesis in Group 3 Medulloblastoma Patient-Derived Xenografts. Sci Rep 8:6913 (2018). PubMed: 29720672
