Key features and details
- Rabbit polyclonal to Cip4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cip4 antibody
See all Cip4 primary antibodies
DescriptionRabbit polyclonal to Cip4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
A region between residues 551 and 601 of human Cip4
- HeLa cytoplasmic or membrane lysate 293T cytoplasmic or membrane lysate Mouse NIH3T3 cytoplasmic or membrane lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72220 was affinity purified using an epitope specific to Cip4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRequired for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
Tissue specificityExpressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
Sequence similaritiesBelongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain.
modificationsTyrosine phosphorylated. Also phosphorylated by PKA.
Cellular localizationCytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.
- Information by UniProt
- Cdc42 interacting protein 4 antibody
- Cdc42 interaction protein 4 long isoform antibody
- Cdc42-interacting protein 4 antibody
All lanes : Anti-Cip4 antibody (ab72220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Detection of Human Cip4 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with an antibody recognising an upstream epitope in lane 1, ab72220 at 3 µg in lane 2 and control IgG in lane 3. Image shows immunoprecipitated Cip4 detected using post IP WB, loading 20% of IP and using ab72220 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab72220 has been referenced in 1 publication.
- Zhu YC et al. Suppression of CIP4/Par6 attenuates TGF-ß1-induced epithelial-mesenchymal transition in NRK-52E cells. Int J Mol Med 40:1165-1171 (2017). PubMed: 28848997