Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)

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Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
  • Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
  • Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1965] to Cip4 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cip4 antibody [EPR1965] - BSA and Azide free
    See all Cip4 primary antibodies

  • Description

    Rabbit monoclonal [EPR1965] to Cip4 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Recent WB re-tests performed by our lab suggest to use a higher dilution for mouse samples. The tested dilutions were 1:500 and 1:2000, were the signal detected was very strong. Human and rat samples worked fine in these dilutions.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Cip4. The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, JAR, TF1, HeLa and HepG2 cell lysates. IHC-P: Human stomach tissue.

  • General notes

    Ab247627 is the carrier-free version of ab108313. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247627 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.

    • Tissue specificity

      Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.

    • Sequence similarities

      Belongs to the FNBP1 family.
      Contains 1 FCH domain.
      Contains 1 REM (Hr1) repeat.
      Contains 1 SH3 domain.

    • Post-translational
      modifications

      Tyrosine phosphorylated. Also phosphorylated by PKA.

    • Cellular localization

      Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cdc42 interacting protein 4 antibody
      • Cdc42 interaction protein 4 long isoform antibody
      • Cdc42-interacting protein 4 antibody
      • CG11341 antibody
      • CG15015 PA antibody
      • Cip 4 antibody
      • CIP4 antibody
      • CIP4_HUMAN antibody
      • DCIP4 antibody
      • hSTP antibody
      • Protein Felic antibody
      • Salt tolerant protein antibody
      • Salt tolerator antibody
      • STOT antibody
      • STP antibody
      • Thyroid hormone receptor interactor 10 antibody
      • Thyroid receptor interacting protein 10 antibody
      • Thyroid receptor-interacting protein 10 antibody
      • TR-interacting protein 10 antibody
      • TRIP 10 antibody
      • TRIP-10 antibody
      • trip10 antibody
      • Truncated Cdc42 interaction protein 4 antibody
      see all

    Images

    • Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
      Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
      All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : TRIP10 knockout HEK293T cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 68 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab108313, the same antibody clone in a different buffer formulation.

      Lanes 1-4: Merged signal (red and green). Green - ab108313 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab108313 Anti-Cip4 antibody [EPR1965] was shown to specifically react with Cip4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266428 (knockout cell lysate ab258251) was used. Wild-type and Cip4 knockout samples were subjected to SDS-PAGE. ab108313 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
      This data was developed using ab108313, the same antibody clone in a different buffer formulation.ab108313 at 1/100 dilution staining Cip4 in paraffin embedded Human stomach tissue. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
    • Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
      Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
      All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : TF1 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 68 kDa



      This data was developed using ab108313, the same antibody clone in a different buffer formulation.

    • Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
      Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247627? Please let us know so that we can cite the reference in this datasheet.

    ab247627 has not yet been referenced specifically in any publications.

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