Recombinant Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
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Overview
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Product name
Anti-Cip4 antibody [EPR1965] - BSA and Azide free
See all Cip4 primary antibodies -
Description
Rabbit monoclonal [EPR1965] to Cip4 - BSA and Azide free -
Host species
Rabbit -
Specificity
Recent WB re-tests performed by our lab suggest to use a higher dilution for mouse samples. The tested dilutions were 1:500 and 1:2000, were the signal detected was very strong. Human and rat samples worked fine in these dilutions.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cip4. The exact sequence is proprietary.
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General notes
Ab247627 is the carrier-free version of ab108313. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247627 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR1965 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab247627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 68 kDa. |
Target
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Function
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL. -
Tissue specificity
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea. -
Sequence similarities
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
Post-translational
modificationsTyrosine phosphorylated. Also phosphorylated by PKA. -
Cellular localization
Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. - Information by UniProt
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Database links
- Entrez Gene: 9322 Human
- Entrez Gene: 106628 Mouse
- Entrez Gene: 116717 Rat
- Omim: 604504 Human
- SwissProt: Q15642 Human
- SwissProt: Q8CJ53 Mouse
- SwissProt: P97531 Rat
- Unigene: 515094 Human
see all -
Alternative names
- Cdc42 interacting protein 4 antibody
- Cdc42 interaction protein 4 long isoform antibody
- Cdc42-interacting protein 4 antibody
see all
Datasheets and documents
References
ab247627 has not yet been referenced specifically in any publications.
