Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Rat Tissue sections (forebrain)
Specification
forebrain
Permeabilization
Yes - triton-100
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-T
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: DONKEY ANTI RABBIT
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
UBQLN4 is not good for IF
this antibody is good for WB
Abcam response
We would like to thank the reviewer for submitting this Abreview. As the product had not yet been tested in IHC with frozen samples we were unsure what the result might be. The user received a discount code for testing the antibody through our AbTrial program and we appreciate the time and effort that the reviewer has put in to testing this product. We welcome other data testing the antibody in IHC-Fr.
Abcam user community
Verified customer
Submitted Feb 02 2015