Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Tissue lysate - whole (HEART)
Specification
HEART
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 62 kDa
Exposure
5 second(s)
Abcam user community
Verified customer
Submitted Jan 30 2015