Key features and details
- Rabbit polyclonal to CIP75
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CIP75 antibody
See all CIP75 primary antibodies
DescriptionRabbit polyclonal to CIP75
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CIP75 aa 1-50. The exact sequence is proprietary.
Database link: Q9NRR5
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241310 was affinity purified using an epitope specific to CIP75 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 64 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityHighly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.
Sequence similaritiesContains 1 UBA domain.
Contains 1 ubiquitin-like domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Endoplasmic reticulum. May associate with the endoplasmic reticulum.
- Information by UniProt
- 28S ribosomal protein S7 antibody
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All lanes : Anti-CIP75 antibody (ab241310) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3 minutes
Lysates prepared using NETM lysis buffer.
CIP75 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241310 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241310 at 0.4 µg/ml.
Lane 1: ab241310 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysate prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241310 has not yet been referenced specifically in any publications.