Anti-CIP75 antibody (ab241310)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CIP75 antibody
See all CIP75 primary antibodies -
Description
Rabbit polyclonal to CIP75 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CIP75 aa 1-50. The exact sequence is proprietary.
Database link: Q9NRR5 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241310 was affinity purified using an epitope specific to CIP75 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 64 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Tissue specificity
Highly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver. -
Sequence similarities
Contains 1 UBA domain.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. Endoplasmic reticulum. May associate with the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 56893 Human
- Omim: 605440 Human
- SwissProt: Q9NRR5 Human
- Unigene: 283739 Human
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Alternative names
- 28S ribosomal protein S7 antibody
- A1U antibody
- A1Up antibody
see all
Images
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Western blot - Anti-CIP75 antibody (ab241310)All lanes : Anti-CIP75 antibody (ab241310) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3 minutesLysates prepared using NETM lysis buffer.
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Immunoprecipitation - Anti-CIP75 antibody (ab241310)CIP75 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241310 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241310 at 0.4 µg/ml.
Lane 1: ab241310 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysate prepared using NETN lysis buffer.
Datasheets and documents
References
ab241310 has not yet been referenced specifically in any publications.
