Product nameAnti-CIP75 antibody
See all CIP75 primary antibodies
DescriptionRabbit polyclonal to CIP75
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CIP75 aa 1-50. The exact sequence is proprietary.
Database link: Q9NRR5
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241310 was affinity purified using an epitope specific to CIP75 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 64 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityHighly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.
Sequence similaritiesContains 1 UBA domain.
Contains 1 ubiquitin-like domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Endoplasmic reticulum. May associate with the endoplasmic reticulum.
- Information by UniProt
- 28S ribosomal protein S7 antibody
- A1U antibody
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All lanes : Anti-CIP75 antibody (ab241310) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3 minutes
Lysates prepared using NETM lysis buffer.
CIP75 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241310 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241310 at 0.4 µg/ml.
Lane 1: ab241310 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysate prepared using NETN lysis buffer.
ab241310 has not yet been referenced specifically in any publications.