Overview

  • Product name

    Anti-CIRP antibody [6E12D5]
    See all CIRP primary antibodies
  • Description

    Mouse monoclonal [6E12D5] to CIRP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CIRP aa 1-90. Expressed in E.coli.
    Database link: Q14011

  • Positive control

    • WB: Recombinant human CIRP (aa1-90) protein; CIRP (aa1-90)-IgG-Fc-transfected HEK-293 cell lysate. IHC-P: Human colon cancer and bladder cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab233711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
IHC-P 1/200 - 1/1000.

Target

  • Function

    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Domain

    Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
  • Post-translational
    modifications

    Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt
  • Database links

  • Alternative names

    • A18 hnRNP antibody
    • A18HNRNP antibody
    • cirbp antibody
    • CIRBP_HUMAN antibody
    • CIRP antibody
    • Cold inducible RNA binding protein antibody
    • Cold-inducible RNA-binding protein antibody
    • Glycine rich RNA binding protein antibody
    • Glycine-rich RNA-binding protein CIRP antibody
    • testicular tissue protein Li 39 antibody
    see all

Images

  • Anti-CIRP antibody [6E12D5] (ab233711) at 1/500 dilution + Recombinant human CIRP (aa1-90) protein

    Developed using the ECL technique.
  • All lanes : Anti-CIRP antibody [6E12D5] (ab233711) at 1/500 dilution

    Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Human CIRP (aa1-90)-IgG-Fc-transfected HEK-293 cell lysate

    Developed using the ECL technique.
  • Paraffin-embedded human colon cancer tissue stained for CIRP using ab233711 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human bladder cancer tissue stained for CIRP using ab233711 at 1/200 dilution in immunohistochemical analysis. DAB staining.

References

ab233711 has not yet been referenced specifically in any publications.

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