Overview

  • Product name

  • Description

    Goat polyclonal to CIRP
  • Host species

    Goat
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Chicken, Cow, Dog, Human, Cynomolgus monkey, Rainbow trout, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human CIRP aa 161-172 (C terminal).
    Sequence:

    C-RDSYDSYATHNE


    Database link: NP_001271.1

  • Positive control

    • Recombinant Human CIRP protein (ab106903) can be used as a positive control in WB. Mouse or Rat testis lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/20.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Domain

    Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
  • Post-translational
    modifications

    Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt
  • Database links

  • Alternative names

    • A18 hnRNP antibody
    • A18HNRNP antibody
    • cirbp antibody
    • CIRBP_HUMAN antibody
    • CIRP antibody
    • Cold inducible RNA binding protein antibody
    • Cold-inducible RNA-binding protein antibody
    • Glycine rich RNA binding protein antibody
    • Glycine-rich RNA-binding protein CIRP antibody
    • testicular tissue protein Li 39 antibody
    see all

Images

  • Anti-CIRP antibody (ab106230) at 0.01 µg/ml + mouse testis lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 19 kDa

  • ab106230 (3.8µg/ml) staining of paraffin embedded Human Testis shows nuclear staining preferentially in type A spermatogonia. Samples steamed antigen retrieval with citrate buffer pH 6 followed by AP-staining.

References

This product has been referenced in:

  • Downie Ruiz Velasco A  et al. Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia. Mol Ther Nucleic Acids 14:329-338 (2019). Read more (PubMed: 30665182) »
  • Luo Y  et al. Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells. Life Sci N/A:N/A (2018). WB . Read more (PubMed: 29857073) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Testis)
Permeabilization
No
Specification
Testis
Fixative
Paraformaldehyde

Kumaraswami Konda

Verified customer

Submitted Sep 26 2017

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Cardiomyocytes)
Total protein in input
30 µg
Immuno-precipitation step
Other - Protein G coated Dynabeads
Specification
Cardiomyocytes

Abcam user community

Verified customer

Submitted Sep 27 2011

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