Key features and details
- Goat polyclonal to CIRP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-CIRP antibody
See all CIRP primary antibodies
DescriptionGoat polyclonal to CIRP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Chicken, Cow, Dog, Human, Cynomolgus monkey, Rainbow trout, Chinese hamster
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
DomainBoth the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
modificationsMethylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
- Information by UniProt
- A18 hnRNP antibody
- A18HNRNP antibody
- cirbp antibody
Anti-CIRP antibody (ab106230) at 0.01 µg/ml + mouse testis lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 19 kDa
ab106230 (3.8µg/ml) staining of paraffin embedded Human Testis shows nuclear staining preferentially in type A spermatogonia. Samples steamed antigen retrieval with citrate buffer pH 6 followed by AP-staining.
ab106230 has been referenced in 4 publications.
- Downie Ruiz Velasco A et al. Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia. Mol Ther Nucleic Acids 14:329-338 (2019). PubMed: 30665182
- Luo Y et al. Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells. Life Sci N/A:N/A (2018). WB . PubMed: 29857073
- Rao M et al. The regulation of CIRBP by transforming growth factor beta during heat shock-induced testicular injury. Andrology N/A:N/A (2018). PubMed: 30461215
- Rao M et al. Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia. Oncotarget 8:85909-85925 (2017). PubMed: 29156766