Key features and details
- Rabbit polyclonal to CIRP
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-CIRP antibody
See all CIRP primary antibodies
DescriptionRabbit polyclonal to CIRP
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide within Human CIRP aa 50-95 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q14011
- WB: Mouse testis whole cell lysate. IHC-P: Mouse brain tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/100 - 1/1000. Predicted molecular weight: 18 kDa.|
FunctionCold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
DomainBoth the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
modificationsMethylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
Cellular localizationNucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
- Information by UniProt
- A18 hnRNP antibody
- A18HNRNP antibody
- cirbp antibody
Anti-CIRP antibody (ab217963) at 1/300 dilution + mouse testis whole cell lysate
HRP conjugated antibody at 1/5000 dilution
Predicted band size: 18 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of mouse brain tissue labeling CIRP with ab217963 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.
ab217963 has not yet been referenced specifically in any publications.