Overview

  • Product name

  • Description

    Rabbit polyclonal to CIRP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CIRP (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: NP_001271.1

  • Positive control

    • Y79 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

Properties

Applications

Our Abpromise guarantee covers the use of ab94999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Domain

    Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
  • Post-translational
    modifications

    Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt
  • Database links

  • Alternative names

    • A18 hnRNP antibody
    • A18HNRNP antibody
    • cirbp antibody
    • CIRBP_HUMAN antibody
    • CIRP antibody
    • Cold inducible RNA binding protein antibody
    • Cold-inducible RNA-binding protein antibody
    • Glycine rich RNA binding protein antibody
    • Glycine-rich RNA-binding protein CIRP antibody
    • testicular tissue protein Li 39 antibody
    see all

Images

  • ICC/IF image of ab94999 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94999 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CIRP antibody (ab94999) at 1/100 dilution + Y79 cell line lysate at 35 µg

    Predicted band size: 19 kDa

References

This product has been referenced in:

  • Ju Lee H  et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun 8:1456 (2017). Read more (PubMed: 29129916) »
  • Zhang Y  et al. Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner. Nucleic Acids Res 44:761-75 (2016). IF . Read more (PubMed: 26673712) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for getting in touch.


The concentration of ab94999 CIRBP antibody lot number GR1103-8  is  0.5 mg/ml.


I am happy to answer any further questions you have.

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