Anti-CISH/CIS antibody (ab88383)
Key features and details
- Mouse polyclonal to CISH/CIS
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CISH/CIS antibody
See all CISH/CIS primary antibodies -
Description
Mouse polyclonal to CISH/CIS -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human CISH/CIS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_659508.1 -
Positive control
- Human spleen lysate. CISH/CIS transfected 293T cell lysate. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
Tissue specificity
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
Post-translational
modificationsAssociation with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa). - Information by UniProt
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Database links
- Entrez Gene: 1154 Human
- Omim: 602441 Human
- SwissProt: Q9NSE2 Human
- Unigene: 655334 Human
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Alternative names
- BACTS2 antibody
- cis 1 antibody
- CIS antibody
see all
Images
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Anti-CISH/CIS antibody (ab88383) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 29 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CISH/CIS antibody (ab88383) at 1 µg/ml
Lane 1 : CISH/CIS transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 29 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of CISH/CIS in HeLa cells using ab88383 at 10µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab88383 has been referenced in 1 publication.
- Clasen BF et al. Gene expression in skeletal muscle after an acute intravenous GH bolus in human subjects: identification of a mechanism regulating ANGPTL4. J Lipid Res 54:1988-97 (2013). PubMed: 23606725