• Product name
  • Description
    Mouse polyclonal to CISH
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human CISH protein (NP_659508.1)

  • Positive control
    • Human spleen lysate. CISH transfected 293T cell lysate. HeLa cells.



Our Abpromise guarantee covers the use of ab88383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use a concentration of 10 µg/ml.


  • Function
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity
    Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Post-translational
    Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
  • Information by UniProt
  • Database links
  • Alternative names
    • BACTS2 antibody
    • cis 1 antibody
    • CIS antibody
    • CIS-1 antibody
    • CIS1 antibody
    • Cish antibody
    • CISH_HUMAN antibody
    • Cytokine inducible inhibitor of signaling type 1B antibody
    • Cytokine inducible SH2 containing protein antibody
    • Cytokine-inducible SH2-containing protein antibody
    • G18 antibody
    • Protein G18 antibody
    • SOCS antibody
    • Suppressor of cytokine signaling antibody
    see all


  • Anti-CISH antibody (ab88383) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size: 29 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-CISH antibody (ab88383) at 1 µg/ml

    Lane 1 : CISH transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 29 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)

  • Immunofluorescence analysis of CISH in HeLa cells using ab88383 at 10µg/ml.


This product has been referenced in:
  • Clasen BF  et al. Gene expression in skeletal muscle after an acute intravenous GH bolus in human subjects: identification of a mechanism regulating ANGPTL4. J Lipid Res 54:1988-97 (2013). Read more (PubMed: 23606725) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us.

I am sorry to hear that the antibody is not working as previously.

As we discussed over the phone, I have arranged for a free of charge replacement with a new lot number (GR89220-1) which you will receiv...

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Thank you for contacting us.

I checked, but we did not unpublish any CISH antibody in the last couple years.
Also, I did not see any changes made to the MW. Our internal notes do not indicate that.

The predicted MWfor human CI...

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