Anti-CISH/CIS antibody (ab192383)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CISH/CIS antibody
See all CISH/CIS primary antibodies -
Description
Rabbit polyclonal to CISH/CIS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human CISH/CIS.
Database link: Q9NSE2 -
Positive control
- MCF-7, mouse brain lysate and PC12 whole cell lysate.
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General notes
This product was previously labelled as CISH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192383 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab192383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
Target
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Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
Tissue specificity
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
Post-translational
modificationsAssociation with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa). - Information by UniProt
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Database links
- Entrez Gene: 1154 Human
- Entrez Gene: 12700 Mouse
- Entrez Gene: 83681 Rat
- Omim: 602441 Human
- SwissProt: Q9NSE2 Human
- SwissProt: Q62225 Mouse
- SwissProt: O70512 Rat
- Unigene: 655334 Human
see all -
Alternative names
- BACTS2 antibody
- cis 1 antibody
- CIS antibody
see all
Images
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Western blot - Anti-CISH/CIS antibody (ab192383)All lanes : Anti-CISH/CIS antibody (ab192383)
Lane 1 : MCF-7 whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : PC12 whole cell lysate
Predicted band size: 29 kDa
Protocols
References
ab192383 has not yet been referenced specifically in any publications.
