Anti-CISH/CIS antibody (ab192383)

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Western blot - Anti-CISH/CIS antibody (ab192383)

    Key features and details

    • Rabbit polyclonal to CISH/CIS
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-CISH/CIS antibody
      See all CISH/CIS primary antibodies

    • Description

      Rabbit polyclonal to CISH/CIS

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Human

    • Immunogen

      Synthetic peptide corresponding to Human CISH/CIS.
      Database link: Q9NSE2

    • Positive control

      • MCF-7, mouse brain lysate and PC12 whole cell lysate.

    • General notes

       This product was previously labelled as CISH

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituents: 49% PBS, 50% Glycerol
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab192383 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab192383 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.

    Target

    • Function

      SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

    • Tissue specificity

      Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Contains 1 SH2 domain.
      Contains 1 SOCS box domain.

    • Post-translational
      modifications

      Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BACTS2 antibody
      • cis 1 antibody
      • CIS antibody
      • CIS-1 antibody
      • CIS1 antibody
      • Cish antibody
      • CISH_HUMAN antibody
      • Cytokine inducible inhibitor of signaling type 1B antibody
      • Cytokine inducible SH2 containing protein antibody
      • Cytokine-inducible SH2-containing protein antibody
      • G18 antibody
      • Protein G18 antibody
      • SOCS antibody
      • Suppressor of cytokine signaling antibody
      see all

    Images

    • Western blot - Anti-CISH/CIS antibody (ab192383)
      Western blot - Anti-CISH/CIS antibody (ab192383)
      All lanes : Anti-CISH/CIS antibody (ab192383)

      Lane 1 : MCF-7 whole cell lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : PC12 whole cell lysate

      Predicted band size: 29 kDa

    References (0)

    Publishing research using ab192383? Please let us know so that we can cite the reference in this datasheet.

    ab192383 has not yet been referenced specifically in any publications.

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