Key features and details
- Mouse polyclonal to CISH/CIS
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CISH/CIS antibody
See all CISH/CIS primary antibodies
DescriptionMouse polyclonal to CISH/CIS
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human CISH/CIS.
Database link: NP_659508.1
- Human spleen lysate. CISH/CIS transfected 293T cell lysate. HeLa cells.
This product was previously labelled as CISH
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityExpressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain.
modificationsAssociation with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
- Information by UniProt
- BACTS2 antibody
- cis 1 antibody
- CIS antibody
Anti-CISH/CIS antibody (ab88383) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 29 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
All lanes : Anti-CISH/CIS antibody (ab88383) at 1 µg/ml
Lane 1 : CISH/CIS transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 29 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of CISH/CIS in HeLa cells using ab88383 at 10µg/ml.
ab88383 has been referenced in 1 publication.
- Clasen BF et al. Gene expression in skeletal muscle after an acute intravenous GH bolus in human subjects: identification of a mechanism regulating ANGPTL4. J Lipid Res 54:1988-97 (2013). PubMed: 23606725