• Product name

    Anti-CISH/CIS antibody - C-terminal
    See all CISH/CIS primary antibodies
  • Description

    Rabbit polyclonal to CISH/CIS - C-terminal
  • Host species

  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Human CISH/CIS aa 241-258 (C terminal).


    Database link: Q9NSE2

  • Positive control

    • Human lung cancer tissue; A431, SMMC, COLO320, HeLa and M231 cell lysates; Rat liver, Rat kidney and Human placenta tissue lysates.
  • General notes

     This product was previously labelled as CISH




Our Abpromise guarantee covers the use of ab191447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 29 kDa.


  • Function

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity

    Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Post-translational

    Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
  • Information by UniProt
  • Database links

  • Alternative names

    • BACTS2 antibody
    • cis 1 antibody
    • CIS antibody
    • CIS-1 antibody
    • CIS1 antibody
    • Cish antibody
    • CISH_HUMAN antibody
    • Cytokine inducible inhibitor of signaling type 1B antibody
    • Cytokine inducible SH2 containing protein antibody
    • Cytokine-inducible SH2-containing protein antibody
    • G18 antibody
    • Protein G18 antibody
    • SOCS antibody
    • Suppressor of cytokine signaling antibody
    see all


  • All lanes : Anti-CISH/CIS antibody - C-terminal (ab191447) at 0.5 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat kidney tissue lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : A431 cell lysate
    Lane 5 : SMMC cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : COLO320 cell lysate
    Lane 8 : M231 cell lysate

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling CISH/CIS with ab191447 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CISH/CIS with ab191447 at 1 μg/ml.


ab191447 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191447.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up