Key features and details
- Rabbit polyclonal to CISH/CIS - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-CISH/CIS antibody - C-terminal
See all CISH/CIS primary antibodies
DescriptionRabbit polyclonal to CISH/CIS - C-terminal
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow
- Human lung cancer tissue; A431, SMMC, COLO320, HeLa and M231 cell lysates; Rat liver, Rat kidney and Human placenta tissue lysates.
This product was previously labelled as CISH
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.45% Sodium chloride, 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 29 kDa.|
FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityExpressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain.
modificationsAssociation with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
- Information by UniProt
- BACTS2 antibody
- cis 1 antibody
- CIS antibody
All lanes : Anti-CISH/CIS antibody - C-terminal (ab191447) at 0.5 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat kidney tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : A431 cell lysate
Lane 5 : SMMC cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : COLO320 cell lysate
Lane 8 : M231 cell lysate
Predicted band size: 29 kDa
Observed band size: 29 kDa
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling CISH/CIS with ab191447 at 1 μg/ml.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CISH/CIS with ab191447 at 1 μg/ml.
ab191447 has not yet been referenced specifically in any publications.