Overview

  • Product name

    Anti-CISH/CIS antibody - C-terminal
    See all CISH/CIS primary antibodies
  • Description

    Rabbit polyclonal to CISH/CIS - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Human CISH/CIS aa 241-258 (C terminal).
    Sequence:

    LPLPRRMADYLRQYPFQL


    Database link: Q9NSE2

  • Positive control

    • Human lung cancer tissue; A431, SMMC, COLO320, HeLa and M231 cell lysates; Rat liver, Rat kidney and Human placenta tissue lysates.
  • General notes

     This product was previously labelled as CISH

     

Properties

Applications

Our Abpromise guarantee covers the use of ab191447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 29 kDa.

Target

  • Function

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity

    Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Post-translational
    modifications

    Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
  • Information by UniProt
  • Database links

  • Alternative names

    • BACTS2 antibody
    • cis 1 antibody
    • CIS antibody
    • CIS-1 antibody
    • CIS1 antibody
    • Cish antibody
    • CISH_HUMAN antibody
    • Cytokine inducible inhibitor of signaling type 1B antibody
    • Cytokine inducible SH2 containing protein antibody
    • Cytokine-inducible SH2-containing protein antibody
    • G18 antibody
    • Protein G18 antibody
    • SOCS antibody
    • Suppressor of cytokine signaling antibody
    see all

Images

  • All lanes : Anti-CISH/CIS antibody - C-terminal (ab191447) at 0.5 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat kidney tissue lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : A431 cell lysate
    Lane 5 : SMMC cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : COLO320 cell lysate
    Lane 8 : M231 cell lysate

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling CISH/CIS with ab191447 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CISH/CIS with ab191447 at 1 μg/ml.

References

ab191447 has not yet been referenced specifically in any publications.

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