Overview

  • Product name

    Anti-CITED1 antibody
  • Description

    Mouse monoclonal to CITED1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment: SMQLQKLNSQ YQGMAAATPG QPGEAGPLQN WDFGAQAGGA ESLSPSAGAQ SPAIIDSDPV DEEVLMSLVV ELGLDRANEL PELWLGQNEF DFTADFPSSC , corresponding to amino acids 94-194 of Human CITED1 with proprietary tag. (NP_004134)

  • Positive control

  • General notes

    This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab87978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 20 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently.

Target

  • Function

    Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner.
  • Tissue specificity

    Expressed only in melanocytes and testis.
  • Sequence similarities

    Belongs to the CITED family.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation changes in a cell cycle-dependent manner and reduces its transcriptional coactivator activity.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm by a nuclear export signal and (NES) in a CRM1-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABCC1 antibody
    • AI316840 antibody
    • AU019144 antibody
    • Cbp/p300-interacting transactivator 1 antibody
    • Cbp/p300-interacting transactivator with GLU/ASP-rich C-terminal domain, 1 antibody
    • Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1 antibody
    • CITE1_HUMAN antibody
    • CITED1 antibody
    • melanocyte specific gene 1 antibody
    • Melanocyte specific protein 1 antibody
    • Melanocyte-specific protein 1 antibody
    • MSG1 antibody
    see all

Images

  • Anti-CITED1 antibody (ab87978) at 5 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size: 20 kDa
    Observed band size: 20 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



    This image was generated using the ascites version of the product.

  • All lanes : Anti-CITED1 antibody (ab87978) at 1 µg/ml

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 20 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

  • Immunoperoxidase of monoclonal antibody ab87978 at 1.2 ug/ml to CITED1 on formalin-fixed paraffin-embedded Human testis.

    This image was generated using the ascites version of the product.

  • Capture ELISA using recombinant protein. Detection limit is approximately 0.03ng/ml as a capture antibody.

    This image was generated using the ascites version of the product.

  • Anti-CITED1 antibody (ab87978) at 5 µg/ml + Recombinant protein used as immunogen at 0.2 µg/ml

    Predicted band size: 20 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Vargas D  et al. Thermogenic capacity of human periaortic adipose tissue is transformed by body weight. PLoS One 13:e0194269 (2018). Read more (PubMed: 29554135) »
  • Zhao H  et al. Melanocyte-specific gene 1 promotes melanoma progression by enhancing the expression of Bcl-2. Oncol Lett 15:2413-2418 (2018). Read more (PubMed: 29434952) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
None
Permeabilization
Yes - 0.3%Tween in PBS
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Dr. Timo Rademakers

Verified customer

Submitted Oct 01 2018

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (kidney)
Specification
kidney
Permeabilization
Yes - Methanol
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 29 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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