Anti-Citrate synthetase antibody [2H8BB6] (ab128564)
Key features and details
- Mouse monoclonal [2H8BB6] to Citrate synthetase
- Suitable for: IP, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-Citrate synthetase antibody [2H8BB6]
See all Citrate synthetase primary antibodies -
Description
Mouse monoclonal [2H8BB6] to Citrate synthetase -
Host species
Mouse -
Tested Applications & Species
Application Species ICC/IF HumanIP Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
Positive control
- Human Liver Mitochondria;
-
General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
-
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
2H8BB6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IP |
Human
|
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
Notes |
---|
IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
-
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
Sequence similarities
Belongs to the citrate synthase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 1431 Human
- Omim: 118950 Human
- SwissProt: O75390 Human
- Unigene: 430606 Human
- Unigene: 728168 Human
-
Alternative names
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
Images
-
Immunoprecipitation using ab128564
Lane 1: Ladder
Lane 2: Human Liver Mitochondria -
Immunofluorescent staining of Citrate synthetase in Hela cells (human) using ab128564.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128564, 5µg/ml) for 2h at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab128564 has been referenced in 2 publications.
- Askeland G et al. Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients. Sci Rep 8:9817 (2018). WB ; Human . PubMed: 29959348
- Rhein VF et al. Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase. FEBS Lett 591:1641-1652 (2017). PubMed: 28391595