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Synthetic peptide corresponding to Human Citrate synthetase aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: O75390
Our Abpromise guarantee covers the use of ab151607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).|
The band observed at 48 kDa could potentially be a cleaved form of Citrate synthetase due to the presence of a 27 amino acid transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151607 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab151607 has not yet been referenced specifically in any publications.
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