Anti-Citrate synthetase antibody (ab211479)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Citrate synthetase antibody
See all Citrate synthetase primary antibodies -
Description
Rabbit polyclonal to Citrate synthetase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human Citrate synthetase (internal sequence).
Database link: O75390 -
Positive control
- Human heart tissue. HepG2 whole cell lysate. Mouse brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: 20% Glycerol, 79% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab211479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
Target
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Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
Sequence similarities
Belongs to the citrate synthase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 100858903 Chicken
- Entrez Gene: 280682 Cow
- Entrez Gene: 1431 Human
- Entrez Gene: 12974 Mouse
- Entrez Gene: 397519 Pig
- Entrez Gene: 170587 Rat
- Entrez Gene: 322339 Zebrafish
- Omim: 118950 Human
see all -
Alternative names
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab211479)Immunohistochemistry of formalin-fixed, paraffin-embedded human heart labeling Citrate synthetase with ab211479 at 10 µg/mL.
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Western blot - Anti-Citrate synthetase antibody (ab211479)Anti-Citrate synthetase antibody (ab211479) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 52 kDa10% SDS-PAGE
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Western blot - Anti-Citrate synthetase antibody (ab211479)Anti-Citrate synthetase antibody (ab211479) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg
Predicted band size: 52 kDa10% SDS-PAGE
Protocols
References
ab211479 has not yet been referenced specifically in any publications.
