Recombinant
RabMAb

Recombinant Anti-Citrate synthetase antibody [EPR8066] (ab129088)

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Overview

  • Product name

    Anti-Citrate synthetase antibody [EPR8066]
    See all Citrate synthetase primary antibodies

  • Description

    Rabbit monoclonal [EPR8066] to Citrate synthetase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human Citrate synthetase (UniProt ID: O75390).

  • Positive control

    • HepG2, Caco-2, 293T, and HeLa cell lysates, Human kidney tissue

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab129088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    Images

    • Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
      Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
      All lanes : Anti-Citrate synthetase antibody [EPR8066] (ab129088) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Caco-2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 51 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)

      ab129088, at a dilution of 1/100, staining Citrate synthetase in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Chen LY  et al. Promotion of mitochondrial energy metabolism during hepatocyte apoptosis in a rat model of acute liver failure. Mol Med Rep 12:5035-41 (2015). WB ; Rat . Read more (PubMed: 26135512) »
    • Zoladz JA  et al. Mechanisms responsible for the acceleration of pulmonary V?O2 on-kinetics in humans after prolonged endurance training. Am J Physiol Regul Integr Comp Physiol 307:R1101-14 (2014). Read more (PubMed: 25163914) »
    See all 2 Publications for this product

    Publishing research using ab129088? Please let us know so that we can cite the reference in this datasheet.

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