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Signal Transduction Metabolism Energy Metabolism
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RecombinantRabMAb

Recombinant Anti-Citrate synthetase antibody [EPR8066] (ab129088)

  • Datasheet
  • SDS
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Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
  • Anti-Citrate synthetase antibody [EPR8066] (ab129088)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8066] to Citrate synthetase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Citrate synthetase antibody [EPR8066]
    See all Citrate synthetase primary antibodies
  • Description

    Rabbit monoclonal [EPR8066] to Citrate synthetase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human Citrate synthetase (UniProt ID: O75390).

  • Positive control

    • HepG2, Caco-2, 293T, and HeLa cell lysates, Human kidney tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8066
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-Citrate synthetase antibody [EPR8066] - BSA and Azide free (ab248292)
  • Assay kits

    • Citrate Synthase Activity Assay Kit (ab119692)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Citrate synthetase protein (ab183252)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Pathway

      Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.
    • Sequence similarities

      Belongs to the citrate synthase family.
    • Cellular localization

      Mitochondrion matrix.
    • Target information above from: UniProt accession O75390 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1431 Human
      • Entrez Gene: 170587 Rat
      • Omim: 118950 Human
      • SwissProt: O75390 Human
      • SwissProt: Q8VHF5 Rat
      • Unigene: 430606 Human
      • Unigene: 728168 Human
      • Unigene: 66581 Rat
      • Alternative names

        • CISY_HUMAN antibody
        • Citrate synthase antibody
        • Citrate synthase, mitochondrial antibody
        • citrate synthetase antibody
        • Cs antibody
        • EC 2.3.3 antibody
        • EC 2.3.3.1 antibody
        see all

      Images

      • Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
        Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
        All lanes : Anti-Citrate synthetase antibody [EPR8066] (ab129088) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : Caco-2 cell lysate
        Lane 3 : 293T cell lysate
        Lane 4 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 51 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)

        ab129088, at a dilution of 1/100, staining Citrate synthetase in paraffin-embedded Human kidney tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-Citrate synthetase antibody [EPR8066] (ab129088)
        Anti-Citrate synthetase antibody [EPR8066] (ab129088)

      Protocols

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab129088? Please let us know so that we can cite the reference in this datasheet.

      ab129088 has been referenced in 2 publications.

      • Chen LY  et al. Promotion of mitochondrial energy metabolism during hepatocyte apoptosis in a rat model of acute liver failure. Mol Med Rep 12:5035-41 (2015). WB ; Rat . PubMed: 26135512
      • Zoladz JA  et al. Mechanisms responsible for the acceleration of pulmonary V?O2 on-kinetics in humans after prolonged endurance training. Am J Physiol Regul Integr Comp Physiol 307:R1101-14 (2014). PubMed: 25163914

      Customer reviews and Q&As

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