RecombinantRabMAb

Recombinant Anti-Citrate synthetase antibody [EPR8066] (ab129088)

Submit a review Submit a question References (2)
Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
  • Anti-Citrate synthetase antibody [EPR8066] (ab129088)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8066] to Citrate synthetase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Citrate synthetase antibody [EPR8066]
    See all Citrate synthetase primary antibodies

  • Description

    Rabbit monoclonal [EPR8066] to Citrate synthetase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide corresponding to residues in Human Citrate synthetase (UniProt ID: O75390).

  • Positive control

    • HepG2, Caco-2, 293T, and HeLa cell lysates, Human kidney tissue

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    Images

    • Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
      Western blot - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
      All lanes : Anti-Citrate synthetase antibody [EPR8066] (ab129088) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Caco-2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 51 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8066] (ab129088)

      ab129088, at a dilution of 1/100, staining Citrate synthetase in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Citrate synthetase antibody [EPR8066] (ab129088)
      Anti-Citrate synthetase antibody [EPR8066] (ab129088)

    References (2)

    Publishing research using ab129088? Please let us know so that we can cite the reference in this datasheet.

    ab129088 has been referenced in 2 publications.

    • Chen LY  et al. Promotion of mitochondrial energy metabolism during hepatocyte apoptosis in a rat model of acute liver failure. Mol Med Rep 12:5035-41 (2015). WB ; Rat . PubMed: 26135512
    • Zoladz JA  et al. Mechanisms responsible for the acceleration of pulmonary V?O2 on-kinetics in humans after prolonged endurance training. Am J Physiol Regul Integr Comp Physiol 307:R1101-14 (2014). PubMed: 25163914

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