Anti-Citrulline antibody [6C2.1] (ab240908)

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Overview

  • Product name

    Anti-Citrulline antibody [6C2.1]
    See all Citrulline primary antibodies

  • Description

    Mouse monoclonal [6C2.1] to Citrulline

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to Citrulline. Synthetic L-Citrulline conjugated to Keyhole Limpet Kemocyanin (KLH).

  • Positive control

    • IHC-P: Mouse colon tissue. WB: Citrulline-BSA.

Properties

Applications

Our Abpromise guarantee covers the use of ab240908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/25.

Target

  • Relevance

    The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (ab240908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (ab240908)

    Formalin-fixed, paraffin-embeded mouse colon tissue stained for Citrulline (Green) using ab240908 at 1/25 dilution for 1 hour at RT in immunohistochemical analysis. Goat Anti-Mouse IgG Alexa Fluor 488 was used as the secondary antibody. The nuclear counterstain is DAPI (Blue).

    (A) DAPI (B) ab240908 (C) Composite

  • Western blot - Anti-Citrulline antibody [6C2.1] (ab240908)
    Western blot - Anti-Citrulline antibody [6C2.1] (ab240908)
    All lanes : Anti-Citrulline antibody [6C2.1] (ab240908) at 1/1000 dilution

    Lane 1 : BSA at 0.5 µg
    Lane 2 : BSA at 2 µg
    Lane 3 : Citrulline-BSA at 0.5 µg
    Lane 4 : Citrulline-BSA at 2 µg

    Secondary
    All lanes : Goat Anti-Mouse IgG HRP at 1:2000 for 60 min at RT

    Developed using the ECL technique.

    Exposure time: 5 minutes

References

ab240908 has not yet been referenced specifically in any publications.

Publishing research using ab240908? Please let us know so that we can cite the reference in this datasheet.

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