Product nameAnti-Citrulline antibody [6C2.1]
See all Citrulline primary antibodies
DescriptionMouse monoclonal [6C2.1] to Citrulline
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Citrulline. Synthetic L-Citrulline conjugated to Keyhole Limpet Kemocyanin (KLH).
- IHC-P: Mouse colon tissue. WB: Citrulline-BSA.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab240908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceThe amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
Formalin-fixed, paraffin-embeded mouse colon tissue stained for Citrulline (Green) using ab240908 at 1/25 dilution for 1 hour at RT in immunohistochemical analysis. Goat Anti-Mouse IgG Alexa Fluor 488 was used as the secondary antibody. The nuclear counterstain is DAPI (Blue).
(A) DAPI (B) ab240908 (C) Composite
All lanes : Anti-Citrulline antibody [6C2.1] (ab240908) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : BSA at 2 µg
Lane 3 : Citrulline-BSA at 0.5 µg
Lane 4 : Citrulline-BSA at 2 µg
All lanes : Goat Anti-Mouse IgG HRP at 1:2000 for 60 min at RT
Developed using the ECL technique.
Exposure time: 5 minutes
ab240908 has not yet been referenced specifically in any publications.