Anti-Citrulline antibody [6C2.1] (ab240908)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (ab240908)
  • Western blot - Anti-Citrulline antibody [6C2.1] (ab240908)

Key features and details

  • Mouse monoclonal [6C2.1] to Citrulline
  • Suitable for: WB, IHC-P
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-Citrulline antibody [6C2.1]
    See all Citrulline primary antibodies

  • Description

    Mouse monoclonal [6C2.1] to Citrulline

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to Citrulline. Synthetic L-Citrulline conjugated to Keyhole Limpet Kemocyanin (KLH).

  • Positive control

    • IHC-P: Mouse colon tissue. WB: Citrulline-BSA.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/25.

Target

  • Relevance

    The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (ab240908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (ab240908)

    Formalin-fixed, paraffin-embeded mouse colon tissue stained for Citrulline (Green) using ab240908 at 1/25 dilution for 1 hour at RT in immunohistochemical analysis. Goat Anti-Mouse IgG Alexa Fluor 488 was used as the secondary antibody. The nuclear counterstain is DAPI (Blue).

    (A) DAPI (B) ab240908 (C) Composite

  • Western blot - Anti-Citrulline antibody [6C2.1] (ab240908)
    Western blot - Anti-Citrulline antibody [6C2.1] (ab240908)
    All lanes : Anti-Citrulline antibody [6C2.1] (ab240908) at 1/1000 dilution

    Lane 1 : BSA at 0.5 µg
    Lane 2 : BSA at 2 µg
    Lane 3 : Citrulline-BSA at 0.5 µg
    Lane 4 : Citrulline-BSA at 2 µg

    Secondary
    All lanes : Goat Anti-Mouse IgG HRP at 1:2000 for 60 min at RT

    Developed using the ECL technique.

    Exposure time: 5 minutes

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240908? Please let us know so that we can cite the reference in this datasheet.

ab240908 has not yet been referenced specifically in any publications.

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