Anti-Citrulline antibody [6C2.1] (ab240908)
Key features and details
- Mouse monoclonal [6C2.1] to Citrulline
- Suitable for: WB, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-Citrulline antibody [6C2.1]
See all Citrulline primary antibodies -
Description
Mouse monoclonal [6C2.1] to Citrulline -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Citrulline. Synthetic L-Citrulline conjugated to Keyhole Limpet Kemocyanin (KLH).
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Positive control
- IHC-P: Mouse colon tissue. WB: Citrulline-BSA.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6C2.1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab240908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000. | |
IHC-P | 1/25. |
Target
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Relevance
The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (ab240908)
Formalin-fixed, paraffin-embeded mouse colon tissue stained for Citrulline (Green) using ab240908 at 1/25 dilution for 1 hour at RT in immunohistochemical analysis. Goat Anti-Mouse IgG Alexa Fluor 488 was used as the secondary antibody. The nuclear counterstain is DAPI (Blue).
(A) DAPI (B) ab240908 (C) Composite
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All lanes : Anti-Citrulline antibody [6C2.1] (ab240908) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : BSA at 2 µg
Lane 3 : Citrulline-BSA at 0.5 µg
Lane 4 : Citrulline-BSA at 2 µg
Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1:2000 for 60 min at RT
Developed using the ECL technique.
Exposure time: 5 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240908 has not yet been referenced specifically in any publications.