Key features and details
- Rabbit polyclonal to Citrulline
- Suitable for: ELISA, ICC
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Citrulline antibody
See all Citrulline primary antibodies
DescriptionRabbit polyclonal to Citrulline
Specificityab100932 reacts specifically with intrapeptidic citrulline independently of the amino acid sequence. It does not react with free citrulline, ornithine or arginine. Citrulline is an un-natural amino-acid and its expression is very low. Factors limiting the detection are the amount of endogenous citrulline within a protein and the accessibility of the antibody to these sites.
Tested applicationsSuitable for: ELISA, ICCmore details
Unsuitable for: WB
Species reactivityReacts with: Species independent
Citrulline coupled to KLH via Glutaraldehyde
- Hypercitrullinated Histones induced in HL60 cells by DMSO +calcium +calcium ionophore A23187
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab100932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
RelevanceThe amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
ELISA data showing the high immuno-reactivity of ab100932 against citrulline due to the high amounts of citrulline conjugated to BSA.
ELISA data demonstrating the specificity of ab100932 to citrulline naturally present in histones (endogenous citrulline).
Immunocytochemical analysis of Human buccal mucosal cells, staining Citrulline with ab100932.
Cells were fixed with acetone followed by 2% glutaraldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 15 hours at 4°C. An undiluted biotinylated anti-rabbit polyclonal IgG was used as the secondary antibody.
ab100932 has been referenced in 10 publications.
- Fonseca Z et al. Pathogenic Entamoeba histolytica, but not Entamoeba dispar, induce neutrophil extracellular trap (NET) formation. J Leukoc Biol 105:1167-1181 (2019). PubMed: 30913315
- Verheul MK et al. Pitfalls in the detection of citrullination and carbamylation. Autoimmun Rev 17:136-141 (2018). PubMed: 29203292
- Hutchinson D et al. Cadmium nanoparticles citrullinate cytokeratins within lung epithelial cells: cadmium as a potential cause of citrullination in chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis 13:441-449 (2018). PubMed: 29430177
- Burbano C et al. Potential Involvement of Platelet-Derived Microparticles and Microparticles Forming Immune Complexes during Monocyte Activation in Patients with Systemic Lupus Erythematosus. Front Immunol 9:322 (2018). PubMed: 29545790
- Fonseca Z et al. Entamoeba histolytica Induce Signaling via Raf/MEK/ERK for Neutrophil Extracellular Trap (NET) Formation. Front Cell Infect Microbiol 8:226 (2018). PubMed: 30023352
- Villar-Vesga J et al. Platelet-derived microparticles generated in vitro resemble circulating vesicles of patients with rheumatoid arthritis and activate monocytes. Cell Immunol N/A:N/A (2018). PubMed: 30538031
- Burbano C et al. Extracellular vesicles are associated with the systemic inflammation of patients with seropositive rheumatoid arthritis. Sci Rep 8:17917 (2018). PubMed: 30559453
- Hutchinson D et al. Carbamylation/citrullination of IgG Fc in bronchiectasis, established RA with bronchiectasis and RA smokers: a potential risk factor for disease. ERJ Open Res 3:N/A (2017). PubMed: 29204430
- Yamakawa M et al. Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis in SKG mice. Clin Exp Immunol 186:177-189 (2016). PubMed: 27465496
- Deplus R et al. Citrullination of DNMT3A by PADI4 regulates its stability and controls DNA methylation. Nucleic Acids Res N/A:N/A (2014). WB . PubMed: 24957603