Question (62309) | Anti-Citrulline antibody (ab100932)
Go to datasheet (ab100932)Do you have an image or protocol for using this antibody in WB?
Abcam community
Verified customer
Asked on Sep 28 2012
Thank you for your inquiry.
The lab did not have a positive control testing WB image, however, they were able to pass along the protocol tested:
The positive control was HL60 cells treated with DMSO or with DMSO and A23187. The histones of these cells were then extracted.
The sample were loaded on a 14% acrylamide SDS PAGE gel.
The transfer was made on a PVDF membrane
The blocking buffer was PBS 1X with 5% BSA
The antibody was used at 1/1000 in a TBST Tween 20 buffer with 1% BSA.
The antibody was incubated overnight at +4°C.
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Answered on Sep 28 2012
