Product nameAnti-Citrulline antibody
See all Citrulline primary antibodies
DescriptionRabbit polyclonal to Citrulline
SpecificityUsing conjugated citrulline glutaraldehyde Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
G = Glutaraldehyde, BSA = Bovine Serum Albumin
Compound Cross-reactivity ratio Citrulline-G-BSA 1 Arginine-G-BSA, Glutamine-G-BSA, Ornithine-G-BSA 1 > 100,000 Conjuaged Citrulline and conjugated Homo-citrulline 1:1000
Tested applicationsSuitable for: ICC/IF, IHC-P, ELISA, IHC-Frmore details
Chemical/ Small Molecule by a Glutaraldehyde linker.
General notesThis polyclonal antibody recognizes only citrulline residues on proteins not free citrulline.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Glycerol
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab6464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/5000 - 1/10000.|
|IHC-Fr||1/1000 - 1/5000.|
RelevanceThe amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of breast DCIS tumor with apoptotic center labeling Citrulline with ab6464 at 1/100 dilution. Tissue was fixed in 10% buffered formalin for 24 hrs then transferred to 70% ethanol until processed and embedded and permeabilized with Tris Buffered Saline with 0.025% Triton X-100. Heat mediated antigen retrieval was performed using 10mM Sodium Citrate Buffer with 0.05% Tween 20 pH 6.0. Tissue was blocked with TBS with 10% normal (secondary species) serum and 1% BSA for 1 hour at 37°C, followed by incubation with Anti-Citrulline antibody (ab6464) in TBS with 1% BSA for 18 hours at 4°C at 1/100 dilution. A polyclonal goat anti rabbit IgG biotin conjugate secondary antibody was used at 1/200 dilution.
Immunofluorescence analysis of THP-1 cells, staining Citrulline with ab6464.
Cells were fixed with paraformaldehyde and blocked with 0.05% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/500 in diluent) for 2 hours at 37°C. ab6717 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) was used to detect staining.
This product has been referenced in:
- Mohamed BM et al. Induction of protein citrullination and auto-antibodies production in murine exposed to nickel nanomaterials. Sci Rep 8:679 (2018). Read more (PubMed: 29330439) »
- Verheul MK et al. Pitfalls in the detection of citrullination and carbamylation. Autoimmun Rev 17:136-141 (2018). Read more (PubMed: 29203292) »