Key features and details
- Rabbit polyclonal to CK1 epsilon
- Suitable for: WB, IHC-P, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CK1 epsilon antibody
See all CK1 epsilon primary antibodies
DescriptionRabbit polyclonal to CK1 epsilon
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from internal sequence of Human CK1 epsilon.
- Human brain cortex neurons; LOVO cell extract; HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 47 kDa.|
|IHC-P||Use a concentration of 15 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
Tissue specificityExpressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Casein kinase 1 epsilon antibody
- Casein kinase I antibody
- Casein kinase I epsilon antibody
All lanes : Anti-CK1 epsilon antibody (ab133713) at 1/500 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with immunizing peptide
Predicted band size: 47 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex neurons labelling CK1 epsilon with ab133713 at 15 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescent analysis of HeLa cells labelling CK1 epsilon with ab133713 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.
ab133713 has not yet been referenced specifically in any publications.