Key features and details
- Rabbit polyclonal to CK1 epsilon
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CK1 epsilon antibody
See all CK1 epsilon primary antibodies
DescriptionRabbit polyclonal to CK1 epsilon
Specificityab70110 detects endogenous levels of total CK1 epsilon protein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal sequence of human CK1 epsilon
- LOVO cell extracts, HeLa cells and human placenta tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70110 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab70110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
Tissue specificityExpressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Casein kinase 1 epsilon antibody
- Casein kinase I antibody
- Casein kinase I epsilon antibody
All lanes : Anti-CK1 epsilon antibody (ab70110) at 1/500 dilution
Lane 1 : LOVO cell extracts
Lane 2 : LOVO cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
ab70110 at 1/500 dilution staining CK1 epsilon in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
ab70110 at 1/50 dilution staining CK1 epsilon in human placenta by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
ab70110 has been referenced in 2 publications.
- Lopez-Guerra JL et al. High casein kinase 1 epsilon levels are correlated with better prognosis in subsets of patients with breast cancer. Oncotarget 6:30343-56 (2015). IHC . PubMed: 26327509
- Scoma HD et al. The De-Ubiquitinylating Enzyme, USP2, Is Associated with the Circadian Clockwork and Regulates Its Sensitivity to Light. PLoS One 6:e25382 (2011). WB ; Human . PubMed: 21966515