Overview

  • Product name

    Anti-CK1 epsilon antibody [AF6C1]
    See all CK1 epsilon primary antibodies
  • Description

    Mouse monoclonal [AF6C1] to CK1 epsilon
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human) with a His tag

  • Positive control

    • 293T cell lysate HeLa cell lysate Ramos cell lysate A431 cell lysate Jurkat cell lysate PC12 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, HEPES
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    AF6C1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IP Use at an assay dependent concentration. Use 1µl/400µl lysate.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • Tissue specificity

    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Casein kinase 1 epsilon antibody
    • Casein kinase I antibody
    • Casein kinase I epsilon antibody
    • Casein kinase I isoform epsilon antibody
    • CKI epsilon antibody
    • CKI-epsilon antibody
    • CKIe antibody
    • CKIepsilon antibody
    • CSNK 1E antibody
    • CSNK1E antibody
    • DBT antibody
    • Doubletime, Drosophila, homolog of antibody
    • EC 2.7.11.1 antibody
    • epsilon isoform antibody
    • HCK IE antibody
    • KC1E antibody
    • KC1E_HUMAN antibody
    • kinase CK1 epsilon antibody
    • MGC 10398 antibody
    see all

Images

  • Anti-CK1 epsilon antibody [AF6C1] (ab82426) at 1/1000 dilution + Human brain tissue lysate - whole at 50 µg

    Secondary
    HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 15 seconds

    See Abreview

  • All lanes : Anti-CK1 epsilon antibody [AF6C1] (ab82426) at 1/2000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Ramos cell lysates
    Lane 3 : A431 cell lysates
    Lane 4 : 293T cell lysates
    Lane 5 : Jurkat cell lysates
    Lane 6 : PC12 cell lysates

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Overlay histogram showing HeLa cells stained with ab82426 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82426, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Beale AD  et al. Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells. J Biol Rhythms 34:144-153 (2019). Read more (PubMed: 30898060) »
  • Wang SK  et al. Fam83h null mice support a neomorphic mechanism for human ADHCAI. Mol Genet Genomic Med 4:46-67 (2016). WB, ICC . Read more (PubMed: 26788537) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% acrylamide gel)
Sample
Human Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 24 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up