Key features and details
- Rabbit polyclonal to CKAP1/TBCB
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CKAP1/TBCB antibody
See all CKAP1/TBCB primary antibodies
DescriptionRabbit polyclonal to CKAP1/TBCB
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human CKAP1/TBCB aa 1-244.
MEVTGVSAPTVTVFISSSLNTFRSEKRYSRSLTIAEFKCKLELLVGSPAS CMELELYGVDDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGARLGEYED VSRVEKYTISQEAYDQRQDTVRSFLKRSKLGRYNEEERAQQEAEAAQRLA EEKAQASSIPVGSRCEVRAAGQSPRRGTVMYVGLTDFKPGYWIGVRYDEP LGKNDGSVNGKRYFECQAKYGAFVKPAVVTVGDFPEEDYGLDEI
Database link: Q99426-1
- WB: HeLa, A431, A549 and Jurkat whole cell lysate; Mouse thymus tissue lysate. IHC-P: Human kidney tissue ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
This product was previously labelled as CKAP1
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionBinds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
Tissue specificityFound in most tissues.
Sequence similaritiesBelongs to the TBCB family.
Contains 1 CAP-Gly domain.
modificationsPhosphorylation by PAK1 is required for normal function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone.
- Information by UniProt
- CG22 antibody
- CKAPI antibody
- Cytoskeleton associated protein 1 antibody
Paraffin-embedded human kidney tissue stained for CKAP1/TBCB with ab237483 at a 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-CKAP1/TBCB antibody (ab237483) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Mouse thymus tissue lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for CKAP1/TBCB (Green) using ab237483 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
CKAP1/TBCB was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using 4 µg of ab237483.
Lane 1: Rabbit control IgG (1 µg) in HeLa whole cell lysate.
Lane 2: ab237483 (4 µg) in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (20 µg input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at a 1/50,000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237483 has not yet been referenced specifically in any publications.