Anti-CKAP2/LB1 antibody (ab198188)
Key features and details
- Rabbit polyclonal to CKAP2/LB1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CKAP2/LB1 antibody
See all CKAP2/LB1 primary antibodies -
Description
Rabbit polyclonal to CKAP2/LB1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein (GST-tag) corresponding to Human CKAP2/LB1. Full length.
Database link: Q8WWK9 -
Positive control
- Human colon cancer and human tonsil tissue.
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General notes
This product was previously labelled as CKAP2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab198188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. |
Target
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Function
Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner. -
Tissue specificity
Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney. -
Sequence similarities
Belongs to the CKAP2 family. -
Developmental stage
Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C). -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules. - Information by UniProt
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Database links
- Entrez Gene: 26586 Human
- Omim: 611569 Human
- SwissProt: Q8WWK9 Human
- Unigene: 444028 Human
- Unigene: 521482 Human
- Unigene: 728722 Human
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Alternative names
- AI461656 antibody
- AW228814 antibody
- CKAP 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP2/LB1 antibody (ab198188)
Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil tissue labeling CKAP2/LB1 with ab198188 at 1/70
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP2/LB1 antibody (ab198188)Immunohistochemical analysis of formalin fixed paraffin embedded human colon cancer labeling CKAP2/LB1 with ab198188 at 1/70
Datasheets and documents
References (1)
ab198188 has been referenced in 1 publication.
- Guo QS et al. Involvement of FAK-ERK2 signaling pathway in CKAP2-induced proliferation and motility in cervical carcinoma cell lines. Sci Rep 7:2117 (2017). IHC-P ; Human . PubMed: 28522860
