Anti-CKAP2/LB1 antibody (ab198188)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CKAP2/LB1 antibody
See all CKAP2/LB1 primary antibodies -
Description
Rabbit polyclonal to CKAP2/LB1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein (GST-tag) corresponding to Human CKAP2/LB1. Full length.
Database link: Q8WWK9 -
Positive control
- Human colon cancer and human tonsil tissue.
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General notes
This product was previously labelled as CKAP2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab198188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. |
Target
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Function
Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner. -
Tissue specificity
Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney. -
Sequence similarities
Belongs to the CKAP2 family. -
Developmental stage
Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C). -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules. - Information by UniProt
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Database links
- Entrez Gene: 26586 Human
- Omim: 611569 Human
- SwissProt: Q8WWK9 Human
- Unigene: 444028 Human
- Unigene: 521482 Human
- Unigene: 728722 Human
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Alternative names
- AI461656 antibody
- AW228814 antibody
- CKAP 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP2/LB1 antibody (ab198188)Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil tissue labeling CKAP2/LB1 with ab198188 at 1/70
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP2/LB1 antibody (ab198188)Immunohistochemical analysis of formalin fixed paraffin embedded human colon cancer labeling CKAP2/LB1 with ab198188 at 1/70
Protocols
Datasheets and documents
References
This product has been referenced in:
- Guo QS et al. Involvement of FAK-ERK2 signaling pathway in CKAP2-induced proliferation and motility in cervical carcinoma cell lines. Sci Rep 7:2117 (2017). IHC-P ; Human . Read more (PubMed: 28522860) »
