Key features and details
- Rabbit polyclonal to CKAP2/LB1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CKAP2/LB1 antibody
See all CKAP2/LB1 primary antibodies
DescriptionRabbit polyclonal to CKAP2/LB1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CKAP2/LB1 (internal sequence). The exact sequence is proprietary.
Database link: Q8WWK9
- WB: Raji and NCI-H929 whole cell extracts. ICC/IF: HeLa cells.
This product was previously labelled as CKAP2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 77 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionPossesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
Tissue specificityAbundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
Sequence similaritiesBelongs to the CKAP2 family.
Developmental stagePresent at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
- Information by UniProt
- AI461656 antibody
- AW228814 antibody
- CKAP 2 antibody
All lanes : Anti-CKAP2/LB1 antibody (ab227214) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 2 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 77 kDa
7.5% SDS-PAGE gel.
Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CKAP2/LB1 (green) using ab227214 at 1/500 dilution in ICC/IF.
Nuclear Counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227214 has not yet been referenced specifically in any publications.