Key features and details
- Rabbit polyclonal to CKAP2/LB1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CKAP2/LB1 antibody
See all CKAP2/LB1 primary antibodies
DescriptionRabbit polyclonal to CKAP2/LB1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
Synthetic peptide corresponding to Human CKAP2/LB1 aa 633-683.
Database link: NP_001091995.1
- HeLa whole cell lysate.
This product was previously labelled as CKAP2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85889 was affinity purified using an epitope specific to CKAP2/LB1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPossesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
Tissue specificityAbundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
Sequence similaritiesBelongs to the CKAP2 family.
Developmental stagePresent at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
- Information by UniProt
- AI461656 antibody
- AW228814 antibody
- CKAP 2 antibody
All lanes : Anti-CKAP2/LB1 antibody (ab85889) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 90 seconds
Detection of CKAP2/LB1 by Western Blot of Immunprecipitate.
ab85889, at 1 µg/ml, staining CKAP2/LB1 in HeLa whole cell lysate immunoprecipitated using ab85889 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 90 seconds.
ab85889 has not yet been referenced specifically in any publications.