Overview

  • Product name

    Anti-CKAP4 antibody [1.1_2F4]
    See all CKAP4 primary antibodies
  • Description

    Mouse monoclonal [1.1_2F4] to CKAP4
  • Host species

    Mouse
  • Specificity

    ab127976 specificity has been determined by protein microarray.
  • Tested applications

    Suitable for: IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Colorectal carcinoma (HCT116) cells.

  • Positive control

    • Transfected HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1.1_2F4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 4 µg/ml. per reaction
ICC/IF 1/100.

Target

  • Post-translational
    modifications

    Reversibly palmitoylated.
  • Cellular localization

    Endoplasmic reticulum-Golgi intermediate compartment membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 63 kDa membrane protein antibody
    • CKAP 4 antibody
    • Ckap4 antibody
    • CKAP4_HUMAN antibody
    • Cytoskeleton associated protein 4 antibody
    • Cytoskeleton-associated protein 4 antibody
    • ERGIC 63 antibody
    • ERGIC63 antibody
    • MGC99554 antibody
    • p63 antibody
    • Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment antibody
    • Type II transmembrane protein p63 antibody
    see all

Images

  • ab127976 at 1/100 staining CKAP4 in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.
  • ab127976 at 2 µg was added to aliquots of cleared cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded.
    Lane 1: Cell Lysate control
    Lane 2: Cell lysate with ab127976
    Lane 3: Cell lysate without ab127976
    Lane 4: V5 tag control

References

ab127976 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab127976.
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