Overview

  • Product name

  • Description

    Rabbit polyclonal to CKAP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 245-573 of CKAP4 (UniProt ID: Q07065).

  • Positive control

    • A549, HeLa, HepG2, HCT116 and MDA-MB-231 whole cell lysate; HeLa cells; CL1-5 xenograft tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab152154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 66 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Post-translational
    modifications

    Reversibly palmitoylated.
  • Cellular localization

    Endoplasmic reticulum-Golgi intermediate compartment membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 63 kDa membrane protein antibody
    • CKAP 4 antibody
    • Ckap4 antibody
    • CKAP4_HUMAN antibody
    • Cytoskeleton associated protein 4 antibody
    • Cytoskeleton-associated protein 4 antibody
    • ERGIC 63 antibody
    • ERGIC63 antibody
    • MGC99554 antibody
    • p63 antibody
    • Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment antibody
    • Type II transmembrane protein p63 antibody
    see all

Images

  • Anti-CKAP4 antibody (ab152154) at 1/10000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 66 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol fixed HeLa cells, labeling CKAP4 using ab152154 at 1/200 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded CL1-5 xenograft tissue, labeling CKAP4 using ab152154 at 1/500 dilution.
  • Anti-CKAP4 antibody (ab152154) at 1/2000 dilution + MDA-MB-231 whole cell lysate at 30 µg with 5% non-fat milk in TBST

    Predicted band size: 66 kDa

References

ab152154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab152154.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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