Anti-CKAP4 antibody (ab245508)
Key features and details
- Rabbit polyclonal to CKAP4
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CKAP4 antibody
See all CKAP4 primary antibodies -
Description
Rabbit polyclonal to CKAP4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human CKAP4 aa 552-602. The exact sequence is proprietary. NP_006816.2
Database link: Q07065 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab245508 was affinity purified using an epitope specific to CKAP4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 3-5 µg/mg of lysate.
|
|
WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
|
Notes |
---|
IP
Use at 3-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
Target
-
Post-translational
modificationsReversibly palmitoylated. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10970 Human
- Entrez Gene: 216197 Mouse
- SwissProt: Q07065 Human
- SwissProt: Q8BMK4 Mouse
- Unigene: 74368 Human
- Unigene: 334999 Mouse
-
Alternative names
- 63 kDa membrane protein antibody
- CKAP 4 antibody
- Ckap4 antibody
see all
Images
-
All lanes : Anti-CKAP4 antibody (ab245508) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 seconds -
CKAP4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245508 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245508 at 1 µg/ml.
Lane 1: ab245508 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 1 second.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab245508 has not yet been referenced specifically in any publications.