Overview

  • Product name

  • Description

    Rabbit polyclonal to CKAP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CKAP4 aa 552-602. The exact sequence is proprietary. NP_006816.2
    Database link: Q07065

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245508 was affinity purified using an epitope specific to CKAP4 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 3-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.

Target

  • Post-translational
    modifications

    Reversibly palmitoylated.
  • Cellular localization

    Endoplasmic reticulum-Golgi intermediate compartment membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 63 kDa membrane protein antibody
    • CKAP 4 antibody
    • Ckap4 antibody
    • CKAP4_HUMAN antibody
    • Cytoskeleton associated protein 4 antibody
    • Cytoskeleton-associated protein 4 antibody
    • ERGIC 63 antibody
    • ERGIC63 antibody
    • MGC99554 antibody
    • p63 antibody
    • Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment antibody
    • Type II transmembrane protein p63 antibody
    see all

Images

  • All lanes : Anti-CKAP4 antibody (ab245508) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 3 seconds
  • CKAP4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245508 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245508 at 1 µg/ml.

    Lane 1: ab245508 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 1 second.

References

ab245508 has not yet been referenced specifically in any publications.

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