Product nameAnti-CKAP4 antibody
See all CKAP4 primary antibodies
DescriptionRabbit polyclonal to CKAP4
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human CKAP4 aa 552-602. The exact sequence is proprietary. NP_006816.2
Database link: Q07065
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245508 was affinity purified using an epitope specific to CKAP4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 3-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane.
- Information by UniProt
- 63 kDa membrane protein antibody
- CKAP 4 antibody
- Ckap4 antibody
All lanes : Anti-CKAP4 antibody (ab245508) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 seconds
CKAP4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245508 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245508 at 1 µg/ml.
Lane 1: ab245508 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
ab245508 has not yet been referenced specifically in any publications.