Anti-CSNK2A1 antibody (ab10466)
Key features and details
- Rabbit polyclonal to CSNK2A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CSNK2A1 antibody
See all CSNK2A1 primary antibodies -
Description
Rabbit polyclonal to CSNK2A1 -
Host species
Rabbit -
Specificity
Reacts with alpha subunit. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human CSNK2A1 aa 1-100.
Database link: 1457 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 432370 Chicken
- Entrez Gene: 282419 Cow
- Entrez Gene: 1457 Human
- Entrez Gene: 12995 Mouse
- Entrez Gene: 116549 Rat
- Entrez Gene: 399319 Xenopus laevis
- Omim: 115440 Human
- SwissProt: P21868 Chicken
see all -
Alternative names
- Casein kinase 2 alpha 1 polypeptide antibody
- Casein kinase 2 alpha antibody
- Casein kinase II alpha 1 antibody
see all
Images
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All lanes : Anti-CSNK2A1 antibody (ab10466) at 1/2500 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 45 kDa -
All lanes : Anti-CSNK2A1 antibody (ab10466) at 1/5000 dilution (LI-COR Blocker (1:1) in PBS + 0.1% Tween for 3 hours)
Lane 1 : Whole cell lysate of HeLa cells
Lane 2 : siRNA to CK2 alpha in HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : An Alexa Fluor®680-conjugated Goat anti-rabit polyclonal at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Blocking Step: 50% LI-COR® Odyssey® Blocking Buffer for 45 minutes at room temperature.
All non-specific bands were very weak.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab10466 has been referenced in 11 publications.
- Caefer DM et al. The Okur-Chung Neurodevelopmental Syndrome Mutation CK2K198R Leads to a Rewiring of Kinase Specificity. Front Mol Biosci 9:850661 (2022). PubMed: 35517865
- Raju P et al. Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice. J Clin Invest 130:5197-5208 (2020). PubMed: 32516134
- Mohl BP & Roy P Hsp90 Chaperones Bluetongue Virus Proteins and Prevents Proteasomal Degradation. J Virol 93:N/A (2019). PubMed: 31375577
- Mohl BP & Roy P Cellular Casein Kinase 2 and Protein Phosphatase 2A Modulate Replication Site Assembly of Bluetongue Virus. J Biol Chem 291:14566-74 (2016). WB ; Human . PubMed: 27226558
- Herhaus L et al. Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Sci Signal 8:ra35 (2015). PubMed: 25872870