RecombinantRabMAb

Recombinant Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)

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Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
  • Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
  • Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11985(2)] to CKLF - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CKLF antibody [EPR11985(2)] - C-terminal
    See all CKLF primary antibodies

  • Description

    Rabbit monoclonal [EPR11985(2)] to CKLF - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human CKLF aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9UBR5

  • Positive control

    • K562, HeLa, Jurkat and THP-1 cell lysates, Paraffin-embedded Human pancreas tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11985(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    CKLF is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. CKLF protein is a potent chemoattractant for neutrophils, monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. CKLF may play important roles in inflammation and in the regeneration of skeletal muscle. Alternatively spliced transcript variants encoding different isoforms have been identified.

  • Cellular localization

    Cell Membrane; Multi-pass membrane protein or Secreted

  • Database links

    see all

  • Alternative names

    • C32 antibody
    • Chemokine-like factor antibody
    • chemokine-like factor 1 antibody
    • chemokine-like factor 2 antibody
    • chemokine-like factor 3 antibody
    • chemokine-like factor 4 antibody
    • CKLF1 antibody
    • CKLF2 antibody
    • CKLF3 antibody
    • CKLF4 antibody
    • CKLFSF2 antibody
    • HSPC224 antibody
    • transmembrane proteolipid antibody
    • UCK-1 antibody
    • UCK1 antibody
    see all

Images

  • Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
    Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
    All lanes : Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
    Western blot - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
    All lanes : Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : THP-1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CKLF with ab180512 at 1/100 dilution. Anti-rabbit HRP secondary antibody was used and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)
    Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512)

References (1)

Publishing research using ab180512? Please let us know so that we can cite the reference in this datasheet.

ab180512 has been referenced in 1 publication.

  • Liu Y  et al. CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC. Clin Cancer Res 25:4141-4154 (2019). PubMed: 30918019

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