Overview

  • Product name

  • Description

    Rabbit polyclonal to CKMT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1 and 224 of Human CKMT2 (UniProt ID: P17540).

  • Positive control

    • 293T, A431, HeLa and HepG2 cell lysates; Cal27 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Tissue specificity

    Sarcomere-specific. Found only in heart and skeletal muscles.
  • Sequence similarities

    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CKMT 2 antibody
    • Basic-type mitochondrial creatine kinase antibody
    • CKMT2 antibody
    • CPK antibody
    • Creatine kinase mitochondrial 2 antibody
    • Creatine kinase mitochondrial 2 (sarcomeric) antibody
    • Creatine kinase S-type antibody
    • creatine kinase S-type, mitochondrial antibody
    • Creatine kinase, sarcomeric mitochondrial antibody
    • KCRS_HUMAN antibody
    • Mib CK antibody
    • Mib-CK antibody
    • mitochondrial antibody
    • OTTHUMP00000147542 antibody
    • S-MtCK antibody
    • Sarcomeric mitochondrial creatine kinase antibody
    • SMTCK antibody
    see all

Images

  • Anti-CKMT2 antibody (ab151450) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling CKMT2 with ab151450 at 1/500 dilution. The lower image was co-stained with Hoescht 33342.
  • Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labeling CKMT2 with ab151450 at 1/500 dilution.

References

ab151450 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151450.
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