Key features and details
- Rabbit polyclonal to CKMT2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CKMT2 antibody
See all CKMT2 primary antibodies
DescriptionRabbit polyclonal to CKMT2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
Recombinant fragment corresponding to a region within amino acids 1 and 224 of Human CKMT2 (UniProt ID: P17540).
- 293T, A431, HeLa and HepG2 cell lysates; Cal27 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Tissue specificitySarcomere-specific. Found only in heart and skeletal muscles.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CKMT 2 antibody
- Basic-type mitochondrial creatine kinase antibody
- CKMT2 antibody
Anti-CKMT2 antibody (ab151450) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells labeling CKMT2 with ab151450 at 1/500 dilution. The lower image was co-stained with Hoescht 33342.
Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labeling CKMT2 with ab151450 at 1/500 dilution.
ab151450 has not yet been referenced specifically in any publications.