Key features and details
- Rabbit polyclonal to CKMT2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CKMT2 antibody
See all CKMT2 primary antibodies
DescriptionRabbit polyclonal to CKMT2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Orangutan
- Human heart tissue; NIH 3T3 cells; A549 cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS is without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Predicted molecular weight: 48 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Tissue specificitySarcomere-specific. Found only in heart and skeletal muscles.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CKMT 2 antibody
- Basic-type mitochondrial creatine kinase antibody
- CKMT2 antibody
All lanes : Anti-CKMT2 antibody (ab189314) at 1/500 dilution
Lane 1 : A549 cell extract
Lane 2 : A549 cell extract with synthesized peptide
Predicted band size: 48 kDa
Immunofluorescent analysis of NIH 3T3 cells labeling CKMT2 with ab189314 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labeling CKMT2 with ab189314 at 10 µg/ml.
ab189314 has been referenced in 1 publication.
- Li C et al. Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis. Int J Biol Macromol 162:11-23 (2020). PubMed: 32531365