Key features and details
- Rabbit polyclonal to CKS1
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CKS1 antibody
DescriptionRabbit polyclonal to CKS1
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from the N terminus of human CKS1.
- Extracts from Jurkat cells, treated with serum (20%, 15mins). HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects a band of <19 kDa (predicted molecular weight: 10 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
Sequence similaritiesBelongs to the CKS family.
- Information by UniProt
- CDC2 associated protein CKS1 antibody
- CDC28 protein kinase 1 antibody
- CDC28 protein kinase 1B antibody
All lanes : Anti-CKS1 antibody (ab72639) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with serum (20%, 15mins)
Lane 2 : Extracts from Jurkat cells, treated
with serum (20%, 15mins) with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 10 kDa
Observed band size: <19 kDa why is the actual band size different from the predicted?
ab72639, at 1/500 dilution, staining CKS1 in HeLa cells by Immunofluorescence in the absence or presence of the immunising peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab72639 has been referenced in 1 publication.
- Zeng Z et al. Downregulation of CKS1B restrains the proliferation, migration, invasion and angiogenesis of retinoblastoma cells through the MEK/ERK signaling pathway. Int J Mol Med 44:103-114 (2019). PubMed: 31115482