Recombinant
RabMAb

Anti-CKS2 antibody [EPR7946(2)] (ab155078)

Overview

  • Product name
    Anti-CKS2 antibody [EPR7946(2)]
    See all CKS2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7946(2)] to CKS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to amino acid residues from Human CKS2 (P33552).

  • Positive control
    • Caco-2, 293T and MOLT4 cell lysates; Human cervical carcinoma and Human kidney tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    Images

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CKS2 with ab155078 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling CKS2 with ab155078 at 1/50 dilution.
    • All lanes : Anti-CKS2 antibody [EPR7946(2)] (ab155078) at 1/1000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 10 kDa

    References

    This product has been referenced in:
    • Li J  et al. Integrative analysis of gene expression profiles reveals specific signaling pathways associated with pancreatic duct adenocarcinoma. Cancer Commun (Lond) 38:13 (2018). WB ; Human . Read more (PubMed: 29764514) »
    • Yu MH  et al. Up-regulated CKS2 promotes tumor progression and predicts a poor prognosis in human colorectal cancer. Am J Cancer Res 5:2708-18 (2015). Read more (PubMed: 26609478) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% gel)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryo)
    Specification
    0-4hr embryo
    Treatment
    control shRNA and cks30A shRNA embryos
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Richelle Sopko

    Verified customer

    Submitted Dec 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up