Anti-CLASP1 antibody [KT67] (ab95372)
Key features and details
- Rat monoclonal [KT67] to CLASP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CLASP1 antibody [KT67]
See all CLASP1 primary antibodies -
Description
Rat monoclonal [KT67] to CLASP1 -
Host species
Rat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human CLASP1 (N terminal).
Database link: Q7Z460 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KT67 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 169 kDa.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 169 kDa. |
Target
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Function
Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. -
Sequence similarities
Belongs to the CLASP family.
Contains 7 HEAT repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. - Information by UniProt
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Database links
- Entrez Gene: 23332 Human
- Omim: 605852 Human
- SwissProt: Q7Z460 Human
- Unigene: 469840 Human
- Unigene: 708183 Human
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Alternative names
- 1700030C23Rik antibody
- 5730583A19Rik antibody
- B130045P17Rik antibody
see all
Images
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All lanes : Anti-CLASP1 antibody [KT67] (ab95372) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CLASP1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 169 kDaLanes 1 - 4: Merged signal (red and green). Green - ab95372 observed at 169 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab95372 was shown to specifically react with CLASP1 in wild-type HAP1 cells as signal was lost in CLASP1 knockout cells. Wild-type and CLASP1 knockout samples were subjected to SDS-PAGE. Ab95372 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab95372 has not yet been referenced specifically in any publications.