• Product name

    Anti-CLASP1 antibody [KT67]
    See all CLASP1 primary antibodies
  • Description

    Rat monoclonal [KT67] to CLASP1
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human CLASP1 (N terminal).



Our Abpromise guarantee covers the use of ab95372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 169 kDa.
ICC/IF 1/100.


  • Function

    Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
  • Sequence similarities

    Belongs to the CLASP family.
    Contains 7 HEAT repeats.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1700030C23Rik antibody
    • 5730583A19Rik antibody
    • B130045P17Rik antibody
    • CLAP1_HUMAN antibody
    • clasp1 antibody
    • CLIP associating protein 1 antibody
    • CLIP associating protein CLASP1 antibody
    • CLIP-associating protein 1 antibody
    • Cytoplasmic linker associated protein 1 antibody
    • Cytoplasmic linker-associated protein 1 antibody
    • DKFZp686D1968 antibody
    • DKFZp686H2039 antibody
    • FLJ33821 antibody
    • FLJ41222 antibody
    • hOrbit1 antibody
    • KIAA0622 antibody
    • MAST1 antibody
    • MGC131895 antibody
    • mKIAA0622 antibody
    • Multiple asters 1 antibody
    • Multiple asters homolog 1 antibody
    • Protein Orbit homolog 1 antibody
    see all


  • All lanes : Anti-CLASP1 antibody [KT67] (ab95372) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CLASP1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 169 kDa

    Lanes 1 - 4: Merged signal (red and green). Green - ab95372 observed at 169 kDa. Red - loading control, ab176560, observed at 50 kDa.

    ab95372 was shown to specifically react with CLASP1 in wild-type HAP1 cells as signal was lost in CLASP1 knockout cells. Wild-type and CLASP1 knockout samples were subjected to SDS-PAGE. Ab95372 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.


ab95372 has not yet been referenced specifically in any publications.

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