Overview

  • Product name
  • Description
    Rabbit polyclonal to Claspin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Claspin encoded within exon 8.

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa).

Target

  • Function
    Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • Sequence similarities
    Belongs to the claspin family.
  • Domain
    The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • Post-translational
    modifications
    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claspin antibody
    • claspin homolog (Xenopus laevis) antibody
    • Claspin homolog antibody
    • CLSPN antibody
    • CLSPN protein antibody
    • CLSPN_HUMAN antibody
    • hClaspin antibody
    • Hu Claspin antibody
    see all

Images

  • Sample: Hela cell lysate (20µg)

    Antibody: Affinity purified Rabbit anti-Claspin (ab3719) used at a 1 / 1000 and 1 / 2000 dilution with (+) or without (-) blocking peptide.

     

    Sample: Hela cell lysate (20µg)

    Antibody: Affinity purified Rabbit anti-Claspin (ab3719) used at a 1 / 1000 and 1 / 2000 dilution with (+) or without (-) blocking peptide.

References

This product has been referenced in:
  • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). Read more (PubMed: 12676962) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

The immunogen is from residues 450 and 500 of human Claspin (GeneID 63967).

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Question
Answer

Unfortunately we are not able to provide the blocking peptide for this antibody.

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Answer

Residues 336 to 526 of human claspin (AF297866_1) are encoded by exon 8. For information on all the exons of Human Claspin, go to http://www.ncbi.nlm.nih.gov/sutils/evv.cgi?contig=NT_079482.1&gene=CLSPN&lid=63967 For information on the exons of other proteins, follow these instructions: Start with Locus Link, LL (http://www.ncbi.nlm.nih.gov/LocusLink/). Put in the protein of interest (POI) or the LocusID number for the POI, I will use human ATM (LocusID: 472) as an example. Scroll down until you see the link to the POI. Hs indicates Homo sapiens. Ce indicates C. elegans. Mm indicates mouse (Mus musculus), etc., etc. Follow the hyperlink for the POI. After LL has loaded the information for that protein, you will see several boxes at the top of the page. PUB is a red box and links to Pubmed citations related to the POI. OMIM is an orange box and links to Online Mendelian Inheritance in Man for the POI, etc. Find the orange/tan MAP hyperlink and follow it. You will get a depiction of the location of the locus for your POI. The information for the POI is highlighted in pink. There are several hyperlinks (OMIM, sv, pr, dl, ev, mm, hm). Follow the ev (evidence viewer) hyperlink. You are now at the Evidence Viewer. Scroll down to see nucleotides for the exons and the translation of the protein. For human ATM, exons 1, 2 and the first part of exon 3 make up the 5' UTR. The ORF starts ~ 30 nucleotides into exon 3. You will note several lines that appear to repeat. The first line is the reference sequence, the lines below are translations of other clones. In most cases these agree. Occasionally, there are discrepencies. For example, in exon 9 of human ATM, one of the sequences has a T to A substitution (the A is shown in red), which changes the codon from that for Tyr to the codon for Asn.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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