• Product name
  • Description
    Rabbit polyclonal to Claspin
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Claspin encoded in part by exons 11, 12 and 13.

  • Positive control
    • HeLa cell lysate.



Our Abpromise guarantee covers the use of ab3720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19124652
IP Use at 1-5 µg/mg of lysate.
WB 1/500 - 1/2500. Detects a band of approximately 175 kDa (predicted molecular weight: 150 kDa).
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • Sequence similarities
    Belongs to the claspin family.
  • Domain
    The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • Post-translational
    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Claspin antibody
    • claspin homolog (Xenopus laevis) antibody
    • Claspin homolog antibody
    • CLSPN antibody
    • CLSPN protein antibody
    • CLSPN_HUMAN antibody
    • hClaspin antibody
    • Hu Claspin antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Claspin with ab3720 at 1/1000 (1µg/ml). Detection: DAB.
  • ab3720 (1/200) staining Claspin in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.

    See Abreview

  • ICC/IF image of ab2861 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2861, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Liang X  et al. Anti-TGF-ß Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria. PLoS One 11:e0155534 (2016). IHC . Read more (PubMed: 27187580) »
  • Ho H  et al. RhoJ regulates melanoma chemoresistance by suppressing pathways that sense DNA damage. Cancer Res 72:5516-28 (2012). Read more (PubMed: 22971344) »
See all 12 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Human Cell lysate - nuclear (HeLa nuclear cell lysate)
HeLa nuclear cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 08 2013


The predicted MW of the protein is 151 kDa for isoform 1. Two other isoforms exist, isoform 2 (144 kDa) and isoform 3 (150 kDa) (see http://www.uniprot.org/uniprot/Q9HAW4), but I am not sure under which conditions you would be able to detect either one and in which samples they might be present. The literature might be able to help in this regard.

As for your WB results, the band should be around 175 - 180 kDa that's where the lab and other customers detected the band (see Abreviews). I cannot find an explanation for the 2 bands around 250 kDa as well as the other bands you see around 130, 100 and 70 kDa. I believe these must be non-specific bands.

Read More
Western blot
Human Cell lysate - whole cell (HEK 293)
Loading amount
20 µg
HEK 293
Gel Running Conditions
Non-reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 07 2011

Human Cell lysate - whole cell (HT29 tumour cell line)
Total protein in input
500 µg
HT29 tumour cell line
4Gy Ionising Radiation
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted Aug 13 2009

Western blot
Human Cell lysate - whole cell (ht29 tumour cell line)
Loading amount
50 µg
ht29 tumour cell line
4Gy Ionising Radiation
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Mr. Paul Eve

Verified customer

Submitted Aug 07 2009

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% TritonX100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted May 28 2009

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