Anti-Claspin antibody (ab3721)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Claspin antibody
See all Claspin primary antibodies -
Description
Rabbit polyclonal to Claspin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.
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Positive control
- HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa). | |
| IP | Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks. -
Sequence similarities
Belongs to the claspin family. -
Domain
The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1. -
Post-translational
modificationsPhosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 63967 Human
- Omim: 605434 Human
- SwissProt: Q9HAW4 Human
- Unigene: 175613 Human
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Alternative names
- Claspin antibody
- claspin homolog (Xenopus laevis) antibody
- Claspin homolog antibody
see all
Images
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Western blot - Anti-Claspin antibody (ab3721)Sample: Hela cell lysate (20
µ g).Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.
Detection: ECL.
Sample: Hela cell lysate (20
µg). Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.
Detection: ECL.
References
This product has been referenced in:
- Jiang K et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). Read more (PubMed: 12676962) »
