Anti-Claspin antibody (ab3721)

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Immunoprecipitation - Anti-Claspin antibody (ab3721)
  • Western blot - Anti-Claspin antibody (ab3721)

Key features and details

  • Rabbit polyclonal to Claspin
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Claspin antibody
    See all Claspin primary antibodies

  • Description

    Rabbit polyclonal to Claspin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla

  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.

  • Positive control

    • HeLa cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

  • Sequence similarities

    Belongs to the claspin family.

  • Domain

    The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

  • Post-translational
    modifications

    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Claspin antibody
      • claspin homolog (Xenopus laevis) antibody
      • Claspin homolog antibody
      • CLSPN antibody
      • CLSPN protein antibody
      • CLSPN_HUMAN antibody
      • hClaspin antibody
      • Hu Claspin antibody
      see all

    Images

    • Immunoprecipitation - Anti-Claspin antibody (ab3721)
      Immunoprecipitation - Anti-Claspin antibody (ab3721)

      Claspin was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3721 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3721 at 0.1µg/mL.

      Lane 1: ab3721 IP in HeLa whole cell lysate.

      Lane 2: ab3721 IP in HeLa whole cell lysate.

      Lane 3: Control IgG in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 10 seconds

    • Western blot - Anti-Claspin antibody (ab3721)
      Western blot - Anti-Claspin antibody (ab3721)
      All lanes : Anti-Claspin antibody (ab3721) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK293T whole cell lysate at 50 µg

      Predicted band size: 150 kDa


      Exposure time: 30 seconds

    References (1)

    Publishing research using ab3721? Please let us know so that we can cite the reference in this datasheet.

    ab3721 has been referenced in 1 publication.

    • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). PubMed: 12676962

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