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    claspin-antibody-ab3721.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other
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Anti-Claspin antibody (ab3721)

  • Datasheet
  • SDS
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Western blot - Anti-Claspin antibody (ab3721)

    Key features and details

    • Rabbit polyclonal to Claspin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-Claspin antibody
      See all Claspin primary antibodies
    • Description

      Rabbit polyclonal to Claspin
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.

    • Positive control

      • HeLa cell lysate.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • Other
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Cycle Inhibitors
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3721 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Chimpanzee
    Gorilla
    Application Abreviews Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
    Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).

    Target

    • Function

      Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
    • Sequence similarities

      Belongs to the claspin family.
    • Domain

      The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
    • Post-translational
      modifications

      Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9HAW4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 63967 Human
      • Omim: 605434 Human
      • SwissProt: Q9HAW4 Human
      • Unigene: 175613 Human
      • Alternative names

        • Claspin antibody
        • claspin homolog (Xenopus laevis) antibody
        • Claspin homolog antibody
        • CLSPN antibody
        • CLSPN protein antibody
        • CLSPN_HUMAN antibody
        • hClaspin antibody
        • Hu Claspin antibody
        see all

      Images

      • Western blot - Anti-Claspin antibody (ab3721)
        Western blot - Anti-Claspin antibody (ab3721)

        Sample: Hela cell lysate (20µg).

        Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.

        Detection: ECL.

        Sample: Hela cell lysate (20µg).

        Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.

        Detection: ECL.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab3721? Please let us know so that we can cite the reference in this datasheet.

      ab3721 has been referenced in 1 publication.

      • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). PubMed: 12676962

      Customer reviews and Q&As

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