Overview

  • Product name

    Anti-Clathrin heavy chain antibody
    See all Clathrin heavy chain primary antibodies
  • Description

    Rabbit polyclonal to Clathrin heavy chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Fr, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
    Predicted to work with: Chicken, Cow, Xenopus laevis, Caenorhabditis elegans, Zebrafish, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Rat Clathrin heavy chain aa 619-638.
    Sequence:

    KAGLLQRALEHFTDLYDIKR

  • Positive control

    • Rat lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/400.
WB 1/1000 - 1/8000. Predicted molecular weight: 192 kDa.
IHC-Fr 1/1000 - 1/4000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

Images

  • ab59710 at 1/1000, overnight, secondary antibody biotinylated goat anti-sheep at 1/3000.

    Figure A is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells.

    Figure B is a higher power image of the staining of the non ciliated bronchiolar cells.

  • ab59710 at 1/100, overnight, secondary antibody donkey anti-rabbit Cy2® at 1/50. Confocal image shows intense staining of non-ciliated bronchiolar cells (Clara cells).

References

This product has been referenced in:

  • Eno C  et al. aura (mid1ip1l) regulates the cytoskeleton at the zebrafish egg-to-embryo transition. Development 143:1585-99 (2016). Read more (PubMed: 26965374) »
  • Garcin PO & Panté N Cell migration is another player of the minute virus of mice infection. Virology 468-470C:150-159 (2014). Mouse . Read more (PubMed: 25173091) »
See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Fruit fly (Drosophila melanogaster) Cell (Larval neuromuscular junctions (neurons))
Specification
Larval neuromuscular junctions (neurons)
Permeabilization
Yes - 0.4% Triton-X-100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 13 2014

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (3-8% Tris-Acetate Gradient Gel (Invitrogen Novex))
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (whole fly)
Specification
whole fly
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 12 2014

Question
Answer

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab59710 in pig. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for pig and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Question
Answer

Thank you for your call today and for letting us know about the trouble with this antibody. I sincerely apologize that this problem was not initially resolved, and if there is anything else that we can do for you please let me know.

Your credit note ID is ***.

If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. Our accounting department can be contacted by email at us.credits@abcam.com or by telephoneat 888-772-2226.

Please let me know if you have any questions or need anything else, and I will be happy to help you further. Have a wonderful day.

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Question
Answer



Nous avons dans notre catalogue des anticorps marqueurs des voies d'internalisation tels que les anti-Clathrin ab2731 (https://www.abcam.com/ab2731) et ab59710 (https://www.abcam.com/ab59710). Malheureusement nous n'avons pas ces anticorps couplés à un fluorochrome.

Nous avons cependant des kits de conjugaison, les kits EasyLink, qui permettent de coupler rapidement et facilement les anticorps primaire. Pour plus d'informations sur ces kits je vous invite à visiter la page suivante : https://www.abcam.com/easylink.

Pour les voies d'internalisation je vous suggère le lien suivant : https://www.abcam.com/index.html?pageconfig=resource&rid=10236&pid=14.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Raji)
Total protein in input
10000 cells
Specification
Raji
Treatment
Goat anti Human IgM F(ab) for 5 minutes
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted May 13 2011

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