Overview

  • Product name

    Anti-Clathrin heavy chain antibody [EPR12235(B)]
    See all Clathrin heavy chain primary antibodies
  • Description

    Rabbit monoclonal [EPR12235(B)] to Clathrin heavy chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human Clathrin heavy chain aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q00610

  • Positive control

    • Hela, SH-SY5Y, A431, 293T cell lysates, Human colonic carcinoma tissue, Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 191 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
    • Sequence similarities

      Belongs to the clathrin heavy chain family.
    • Cellular localization

      Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • CHC17 antibody
      • Clathrin heavy chain antibody
      • Clathrin heavy chain 1 antibody
      • Clathrin heavy chain 2 antibody
      • Clathrin heavy chain like 1 antibody
      • Clathrin heavy chain on chromosome 17 antibody
      • Clathrin heavy polypeptide antibody
      • CLH 17 antibody
      • CLH 22 antibody
      • CLH-17 antibody
      • CLH1_HUMAN antibody
      • CLH17 antibody
      • CLH22 antibody
      • CLTC antibody
      • CLTCL antibody
      • CLTCL1 antibody
      • CLTCL2 antibody
      • CLTD antibody
      • Hc antibody
      • KIAA0034 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958) at 1/10000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 191 kDa

    References

    This product has been referenced in:

    • Bridgewater RE  et al. Extracellular matrix promotes clathrin-dependent endocytosis of prolactin and STAT5 activation in differentiating mammary epithelial cells. Sci Rep 7:4572 (2017). WB . Read more (PubMed: 28676702) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172958.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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