Overview

  • Product name
    Anti-Clathrin heavy chain antibody [TD.1]
    See all Clathrin heavy chain primary antibodies
  • Description
    Mouse monoclonal [TD.1] to Clathrin heavy chain
  • Host species
    Mouse
  • Specificity
    This antibody reacts with clathrin heavy chains (CHC).
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    This antibody was raised using the globular N terminal domain of clathrin heavy chain.

  • Positive control
    • Methanol fixed HeLa cells for IF.

Properties

Applications

Our Abpromise guarantee covers the use of ab24578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 21571834
WB 1/250. Predicted molecular weight: 192 kDa.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
    • Sequence similarities
      Belongs to the clathrin heavy chain family.
    • Cellular localization
      Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
      • Alternative names
        • CHC17 antibody
        • Clathrin heavy chain antibody
        • Clathrin heavy chain 1 antibody
        • Clathrin heavy chain 2 antibody
        • Clathrin heavy chain like 1 antibody
        • Clathrin heavy chain on chromosome 17 antibody
        • Clathrin heavy polypeptide antibody
        • CLH 17 antibody
        • CLH 22 antibody
        • CLH-17 antibody
        • CLH1_HUMAN antibody
        • CLH17 antibody
        • CLH22 antibody
        • CLTC antibody
        • CLTCL antibody
        • CLTCL1 antibody
        • CLTCL2 antibody
        • CLTD antibody
        • Hc antibody
        • KIAA0034 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
        Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
        ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.

      References

      This product has been referenced in:
      • Kitahara H  et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . Read more (PubMed: 21571834) »
      • Harel A  et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). Read more (PubMed: 18182011) »

      See all 5 Publications for this product

      Publishing research using ab24578? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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