The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21571834
1/250. Predicted molecular weight: 192 kDa.
Use at an assay dependent concentration.
Is unsuitable for IP.
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
Belongs to the clathrin heavy chain family.
Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
Kitahara H et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res9:688-701 (2011).
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