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Signal Transduction Protein Trafficking Vesicle Transport Coat Proteins
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Anti-Clathrin heavy chain antibody [TD.1] (ab24578)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)

    Key features and details

    • Mouse monoclonal [TD.1] to Clathrin heavy chain
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

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    Secondary
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    Overview

    • Product name

      Anti-Clathrin heavy chain antibody [TD.1]
      See all Clathrin heavy chain primary antibodies
    • Description

      Mouse monoclonal [TD.1] to Clathrin heavy chain
    • Host species

      Mouse
    • Specificity

      This antibody reacts with clathrin heavy chains (CHC).
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Immunogen

      This antibody was raised using the globular N terminal domain of clathrin heavy chain.

    • Positive control

      • Methanol fixed HeLa cells for IF.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.03% Thimerosal (merthiolate)
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      TD.1
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Coat Proteins
      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Caveolae and Clathrin

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Clathrin heavy chain protein (ab112271)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab24578 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
    • Sequence similarities

      Belongs to the clathrin heavy chain family.
    • Cellular localization

      Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession Q00610 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1213 Human
      • Omim: 118955 Human
      • SwissProt: P53675 Human
      • SwissProt: Q00610 Human
      • Unigene: 491351 Human
      • Alternative names

        • CHC17 antibody
        • Clathrin heavy chain antibody
        • Clathrin heavy chain 1 antibody
        • Clathrin heavy chain 2 antibody
        • Clathrin heavy chain like 1 antibody
        • Clathrin heavy chain on chromosome 17 antibody
        • Clathrin heavy polypeptide antibody
        • CLH 17 antibody
        • CLH 22 antibody
        • CLH-17 antibody
        • CLH1_HUMAN antibody
        • CLH17 antibody
        • CLH22 antibody
        • CLTC antibody
        • CLTCL antibody
        • CLTCL1 antibody
        • CLTCL2 antibody
        • CLTD antibody
        • Hc antibody
        • KIAA0034 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
        Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
        ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (6)

      Publishing research using ab24578? Please let us know so that we can cite the reference in this datasheet.

      ab24578 has been referenced in 6 publications.

      • Vassilopoulos S  et al. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol 205:377-93 (2014). PubMed: 24798732
      • Kitahara H  et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . PubMed: 21571834
      • Harel A  et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). PubMed: 18182011
      • Praznikar ZJ  et al. A presynaptically toxic secreted phospholipase A2 is internalized into motoneuron-like cells where it is rapidly translocated into the cytosol. Biochim Biophys Acta 1783:1129-39 (2008). PubMed: 18261469
      • Bushlin I  et al. Clathrin assembly protein AP180 and CALM differentially control axogenesis and dendrite outgrowth in embryonic hippocampal neurons. J Neurosci 28:10257-71 (2008). PubMed: 18842885
      • Borlido J  et al. Clathrin is spindle-associated but not essential for mitosis. PLoS ONE 3:e3115 (2008). WB ; Human . PubMed: 18769625

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