Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
- Datasheet
- References (5)
- Protocols
Overview
-
Product name
Anti-Clathrin heavy chain antibody [TD.1]
See all Clathrin heavy chain primary antibodies -
Description
Mouse monoclonal [TD.1] to Clathrin heavy chain -
Host species
Mouse -
Specificity
This antibody reacts with clathrin heavy chains (CHC). -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Cow, Human -
Immunogen
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
-
Positive control
- Methanol fixed HeLa cells for IF.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Thimerosal (merthiolate) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
TD.1 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab24578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 21571834 | |
| WB | 1/250. Predicted molecular weight: 192 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
Application notes
Is unsuitable for IP.
Target
-
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. -
Sequence similarities
Belongs to the clathrin heavy chain family. -
Cellular localization
Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 281080 Cow
- Entrez Gene: 1213 Human
- Omim: 118955 Human
- SwissProt: P49951 Cow
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- Unigene: 491351 Human
-
Alternative names
- CHC17 antibody
- Clathrin heavy chain antibody
- Clathrin heavy chain 1 antibody
see all
Images
-
![Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)](https://www.abcam.com/ps/products/24/ab24578/Images/ab24578-35349-anti-clathrin-heavy-chain-antibody-td1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
Protocols
References
This product has been referenced in:
- Kitahara H et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . Read more (PubMed: 21571834) »
- Harel A et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). Read more (PubMed: 18182011) »
