Key features and details
- Mouse monoclonal [TD.1] to Clathrin heavy chain
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Clathrin heavy chain antibody [TD.1]
See all Clathrin heavy chain primary antibodies
DescriptionMouse monoclonal [TD.1] to Clathrin heavy chain
SpecificityThis antibody reacts with clathrin heavy chains (CHC).
Tested Applications & Species
Application Species ICC/IFHuman
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
- Methanol fixed HeLa cells for IF.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Thimerosal (merthiolate)
Concentration information loading...
PurityProtein G purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
Sequence similaritiesBelongs to the clathrin heavy chain family.
Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CHC17 antibody
- Clathrin heavy chain antibody
- Clathrin heavy chain 1 antibody
ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
ab24578 has been referenced in 6 publications.
- Vassilopoulos S et al. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol 205:377-93 (2014). PubMed: 24798732
- Kitahara H et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . PubMed: 21571834
- Harel A et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). PubMed: 18182011
- Praznikar ZJ et al. A presynaptically toxic secreted phospholipase A2 is internalized into motoneuron-like cells where it is rapidly translocated into the cytosol. Biochim Biophys Acta 1783:1129-39 (2008). PubMed: 18261469
- Bushlin I et al. Clathrin assembly protein AP180 and CALM differentially control axogenesis and dendrite outgrowth in embryonic hippocampal neurons. J Neurosci 28:10257-71 (2008). PubMed: 18842885
- Borlido J et al. Clathrin is spindle-associated but not essential for mitosis. PLoS ONE 3:e3115 (2008). WB ; Human . PubMed: 18769625